Detailed information of TA system TA157713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1401921..1402758
• Replicon: chromosome
• Accession: NZ_CP053751
• Organism: Shigella sonnei strain 506

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: COO90_RS06780
• Protein ID: WP_000227784.1
• Coordinates: 1401921..1402463 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: COO90_RS06785
• Protein ID: WP_001297137.1
• Coordinates: 1402447..1402758 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COO90_RS06750 (1396985)	1396985..1397476	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
COO90_RS06755 (1397604)	1397604..1398968	-	1365	WP_001000971.1	MFS transporter	-
COO90_RS06760 (1399087)	1399087..1399784	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
COO90_RS06765 (1400153)	1400153..1400551	+	399	Protein_1309	SEL1-like repeat protein	-
COO90_RS06775 (1401299)	1401299..1401865	+	567	Protein_1311	SEL1-like repeat protein	-
COO90_RS06780 (1401921)	1401921..1402463	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
COO90_RS06785 (1402447)	1402447..1402758	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
COO90_RS06790 (1402943)	1402943..1403833	-	891	WP_000971327.1	heme o synthase	-
COO90_RS06795 (1403845)	1403845..1404174	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
COO90_RS06800 (1404174)	1404174..1404788	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
COO90_RS06805 (1404778)	1404778..1406769	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
COO90_RS06810 (1406791)	1406791..1407738	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T157713 WP_000227784.1 NZ_CP053751:c1402463-1401921 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T157713 NZ_CP069451:c907801-907460 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT157713 WP_001297137.1 NZ_CP053751:c1402758-1402447 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT157713 NZ_CP069451:907796-907872 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3