Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 716046..716543 Replicon chromosome
Accession NZ_CP053751
Organism Shigella sonnei strain 506

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag COO90_RS03410 Protein ID WP_001173343.1
Coordinates 716370..716543 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag COO90_RS03405 Protein ID WP_000398619.1
Coordinates 716046..716342 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COO90_RS03385 (712777) 712777..713496 - 720 WP_000611285.1 two-component system response regulator DcuR -
COO90_RS03390 (713493) 713493..715124 - 1632 WP_001216495.1 sensor histidine kinase -
COO90_RS03395 (715305) 715305..715535 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
COO90_RS03400 (715547) 715547..715819 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
COO90_RS03405 (716046) 716046..716342 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
COO90_RS03410 (716370) 716370..716543 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
COO90_RS03415 (716662) 716662..718179 - 1518 WP_001295086.1 lysine--tRNA ligase -
COO90_RS03420 (718416) 718416..719873 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
COO90_RS03425 (719932) 719932..720189 - 258 Protein_656 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T157711 WP_001173343.1 NZ_CP053751:716370-716543 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T157711 NZ_CP069451:599759-599932 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT157711 WP_000398619.1 NZ_CP053751:716046-716342 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT157711 NZ_CP069451:599435-599731 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References