Detailed information of TA system TA157701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/ParD(antitoxin)
• Location: 2624333..2625331
• Replicon: chromosome
• Accession: NZ_CP053749
• Organism: Mycobacterium avium subsp. paratuberculosis S397

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: X7VT18
• Locus tag: MAPS_RS12380
• Protein ID: WP_003876749.1
• Coordinates: 2624333..2624911 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: MAPS_RS12385
• Protein ID: WP_003876750.1
• Coordinates: 2624942..2625331 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAPS_RS12355	2619886..2621106	-	1221	WP_010948702.1	IS110 family transposase	-
MAPS_RS12360	2621426..2621776	+	351	WP_003878038.1	DoxX family protein	-
MAPS_RS12365	2621790..2622590	-	801	WP_003876746.1	nuclear transport factor 2 family protein	-
MAPS_RS12370	2622590..2623150	-	561	WP_003878040.1	pyrazinamidase PncA	-
MAPS_RS12375	2623261..2624328	+	1068	WP_003876748.1	DoxX family membrane protein	-
MAPS_RS12380	2624333..2624911	-	579	WP_003876749.1	zeta toxin family protein	Toxin
MAPS_RS12385	2624942..2625331	-	390	WP_003876750.1	hypothetical protein	Antitoxin
MAPS_RS12390	2625412..2625906	-	495	WP_010949355.1	gluconokinase	-
MAPS_RS12395	2625903..2627900	-	1998	WP_003876752.1	phosphotransferase	-
MAPS_RS12400	2627919..2629436	-	1518	WP_003876753.1	carboxylesterase/lipase family protein	-
MAPS_RS12405	2629516..2630196	+	681	WP_010949357.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2619886..2621085	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (5-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21034.18 Da
• Isoelectric Point: 10.6678

>T157701 WP_003876749.1 NZ_CP053749:c2624911-2624333 [Mycobacterium avium subsp. paratuberculosis S397]
MERLDVVVGPNGAGKSTFIALTLAPLLPGSVVVNADEIARQRWPQDPASHAYDAAKVAADTRAKLIDLGRSFIAETVFSH
PSKLDLLHAARRAGFTVVLHVLLIPEDLAVERVRHRVRAGGHDVPETKIRERHRRLWTPVAEAMTLADLATGYDNSRLRG
PRVVARLSGGLTVGAVDWPAWAPETLRSRWPV

Nucleotide

• Length: 579 bp

>T157701 NZ_CP069447:4193342-4193719 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13569.99 Da
• Isoelectric Point: 5.6717

>AT157701 WP_003876750.1 NZ_CP053749:c2625331-2624942 [Mycobacterium avium subsp. paratuberculosis S397]
MAEAVDRVTRVASDLMDSAAAEGARQSRSAKQQLDHWARVGRAVSSQHTASRRRVEAALAGRLSTAELTAEEGVVFNAEI
SAAIDESLARTNYGATLAGQGVTTVALNDDGEIVEHRPDGTAVVLAGRR

Nucleotide

• Length: 390 bp

>AT157701 NZ_CP069447:4192921-4193295 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0E2W6B4