Detailed information of TA system TA157700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1214978..1215542
• Replicon: chromosome
• Accession: NZ_CP053749
• Organism: Mycobacterium avium subsp. paratuberculosis S397

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: MAPS_RS06035
• Protein ID: WP_003875391.1
• Coordinates: 1215171..1215542 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7L5MGM2
• Locus tag: MAPS_RS06030
• Protein ID: WP_003875390.1
• Coordinates: 1214978..1215184 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAPS_RS06010	1210231..1211229	+	999	WP_003875386.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MAPS_RS06015	1211253..1212461	-	1209	WP_010949696.1	hypothetical protein	-
MAPS_RS06020	1212592..1213665	+	1074	WP_009975391.1	redox-regulated ATPase YchF	-
MAPS_RS06025	1213865..1214809	+	945	WP_003875389.1	HNH endonuclease	-
MAPS_RS06030	1214978..1215184	+	207	WP_003875390.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MAPS_RS06035	1215171..1215542	+	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAPS_RS06040	1215642..1216082	+	441	WP_003875392.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
MAPS_RS06045	1216138..1216413	+	276	WP_003875393.1	hypothetical protein	-
MAPS_RS06050	1216515..1216913	+	399	WP_003875394.1	VOC family protein	-
MAPS_RS06055	1216980..1217363	+	384	WP_003875395.1	hypothetical protein	-
MAPS_RS06060	1217433..1217786	+	354	WP_003875396.1	hypothetical protein	-
MAPS_RS06065	1217943..1218467	+	525	WP_033713819.1	glycoside hydrolase	-
MAPS_RS06070	1218880..1219204	+	325	Protein_1197	antibiotic biosynthesis monooxygenase	-
MAPS_RS06075	1219209..1219892	-	684	WP_003875399.1	hypothetical protein	-
MAPS_RS06080	1220010..1220207	+	198	WP_016706054.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T157700 WP_003875391.1 NZ_CP053749:1215171-1215542 [Mycobacterium avium subsp. paratuberculosis S397]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T157700 NZ_CP069447:3888328-3888546 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7231.22 Da
• Isoelectric Point: 11.0634

>AT157700 WP_003875390.1 NZ_CP053749:1214978-1215184 [Mycobacterium avium subsp. paratuberculosis S397]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Nucleotide

• Length: 207 bp

>AT157700 NZ_CP069447:3887928-3888302 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E2WB37

Antitoxin

• AlphaFold DB: A0A7L5MGM2