Detailed information of TA system TA157695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4824326..4825583
• Replicon: chromosome
• Accession: NZ_CP053747
• Organism: Pseudomonas aeruginosa strain Pae1255-NDM1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HJ571_RS22535
• Protein ID: WP_003449003.1
• Coordinates: 4824326..4825081 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HJ571_RS22540
• Protein ID: WP_003086329.1
• Coordinates: 4825215..4825583 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ571_RS22525 (HJ571_22555)	4819499..4822042	+	2544	WP_034037613.1	acyl-homoserine-lactone acylase QuiP	-
HJ571_RS22530 (HJ571_22560)	4822197..4823558	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
HJ571_RS22535 (HJ571_22565)	4824326..4825081	-	756	WP_003449003.1	RES family NAD+ phosphorylase	Toxin
HJ571_RS22540 (HJ571_22570)	4825215..4825583	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HJ571_RS22545 (HJ571_22575)	4825656..4826942	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
HJ571_RS22550 (HJ571_22580)	4826998..4828587	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
HJ571_RS22555 (HJ571_22585)	4828666..4829142	+	477	WP_003112531.1	hypothetical protein	-
HJ571_RS22560 (HJ571_22590)	4829145..4829420	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28292.45 Da
• Isoelectric Point: 7.7294

>T157695 WP_003449003.1 NZ_CP053747:c4825081-4824326 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T157695 NZ_CP069447:c2242406-2242032 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT157695 WP_003086329.1 NZ_CP053747:c4825583-4825215 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT157695 NZ_CP069447:c2242863-2242495 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7