Detailed information of TA system TA157694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3828423..3829644
• Replicon: chromosome
• Accession: NZ_CP053747
• Organism: Pseudomonas aeruginosa strain Pae1255-NDM1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: HJ571_RS17790
• Protein ID: WP_003102740.1
• Coordinates: 3829066..3829644 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HJ571_RS17785
• Protein ID: WP_003088112.1
• Coordinates: 3828423..3828977 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ571_RS17760 (HJ571_17785)	3823991..3824542	+	552	WP_003102727.1	XRE family transcriptional regulator	-
HJ571_RS17765 (HJ571_17790)	3824539..3824937	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HJ571_RS17770 (HJ571_17795)	3824950..3825273	-	324	WP_034038313.1	multidrug efflux SMR transporter	-
HJ571_RS17775 (HJ571_17800)	3825604..3826065	+	462	WP_034038312.1	(2Fe-2S)-binding protein	-
HJ571_RS17780 (HJ571_17805)	3826070..3828265	+	2196	WP_003122807.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HJ571_RS17785 (HJ571_17810)	3828423..3828977	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
HJ571_RS17790 (HJ571_17815)	3829066..3829644	+	579	WP_003102740.1	HD domain-containing protein	Toxin
HJ571_RS17795 (HJ571_17820)	3829753..3830940	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HJ571_RS17800 (HJ571_17825)	3830930..3833101	-	2172	WP_034038311.1	type I secretion system permease/ATPase	-
HJ571_RS17805 (HJ571_17830)	3833091..3834368	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T157694 WP_003102740.1 NZ_CP053747:3829066-3829644 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T157694 NZ_CP069447:2230064-2230318 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT157694 WP_003088112.1 NZ_CP053747:3828423-3828977 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT157694 NZ_CP069447:2229816-2230067 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07