Detailed information of TA system TA157693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1972129..1973637
• Replicon: chromosome
• Accession: NZ_CP053747
• Organism: Pseudomonas aeruginosa strain Pae1255-NDM1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HJ571_RS09300
• Protein ID: WP_012613851.1
• Coordinates: 1972129..1972716 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HJ571_RS09305
• Protein ID: WP_012613852.1
• Coordinates: 1972783..1973637 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ571_RS09295 (HJ571_09320)	1968384..1971863	-	3480	WP_023115144.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
HJ571_RS09300 (HJ571_09325)	1972129..1972716	+	588	WP_012613851.1	GNAT family protein	Toxin
HJ571_RS09305 (HJ571_09330)	1972783..1973637	+	855	WP_012613852.1	AraC family transcriptional regulator	Antitoxin
HJ571_RS09310 (HJ571_09335)	1973734..1975899	+	2166	WP_003091606.1	TonB-dependent receptor family protein	-
HJ571_RS09315 (HJ571_09340)	1976074..1977948	+	1875	WP_003117607.1	MFS transporter	-
HJ571_RS09320 (HJ571_09345)	1978009..1978218	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T157693 WP_012613851.1 NZ_CP053747:1972129-1972716 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAVLEASFTG

Nucleotide

• Length: 588 bp

>T157693 NZ_CP069447:2229816-2230067 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31959.27 Da
• Isoelectric Point: 7.3276

>AT157693 WP_012613852.1 NZ_CP053747:1972783-1973637 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLPVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT157693 NZ_CP069447:2230064-2230318 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA