Detailed information of TA system TA157688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 409950..410448
• Replicon: chromosome
• Accession: NZ_CP053745
• Organism: Vibrio cholerae strain SA3G

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HPY04_RS15690
• Protein ID: WP_032469509.1
• Coordinates: 409950..410216 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: HPY04_RS15695
• Protein ID: WP_000643598.1
• Coordinates: 410203..410448 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY04_RS15645	405238..405333	+	96	WP_175249424.1	DUF645 family protein	-
HPY04_RS15650	405543..406001	+	459	WP_032473158.1	GNAT family N-acetyltransferase	-
HPY04_RS15655	406318..406497	+	180	WP_050547145.1	DUF645 family protein	-
HPY04_RS15660	406646..406807	+	162	Protein_398	DUF3709 domain-containing protein	-
HPY04_RS15665	406843..406995	+	153	WP_175242275.1	DUF3709 domain-containing protein	-
HPY04_RS15670	407459..407668	+	210	WP_175249665.1	DUF645 family protein	-
HPY04_RS15675	407879..408262	+	384	WP_175249425.1	hypothetical protein	-
HPY04_RS15680	409221..409350	+	130	Protein_402	DUF3265 domain-containing protein	-
HPY04_RS15685	409558..409713	+	156	WP_175249666.1	DUF645 family protein	-
HPY04_RS15690	409950..410216	+	267	WP_032469509.1	BrnT family toxin	Toxin
HPY04_RS15695	410203..410448	+	246	WP_000643598.1	hypothetical protein	Antitoxin
HPY04_RS15700	410595..410966	+	372	WP_032473159.1	nucleotide pyrophosphohydrolase	-
HPY04_RS15705	411264..411443	+	180	WP_148508195.1	DUF645 family protein	-
HPY04_RS15710	411657..412208	+	552	WP_175249426.1	sugar O-acetyltransferase	-
HPY04_RS15715	412368..412850	+	483	WP_175249427.1	GNAT family N-acetyltransferase	-
HPY04_RS15720	413014..413784	+	771	WP_175249428.1	alpha/beta hydrolase	-
HPY04_RS15725	414253..414363	+	111	WP_080284294.1	DUF3265 domain-containing protein	-
HPY04_RS15730	414373..414861	+	489	WP_175249429.1	dual specificity protein phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	336942..428561	91619
	-	inside	Integron	-	-	388738..428561	39823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10079.34 Da
• Isoelectric Point: 7.3171

>T157688 WP_032469509.1 NZ_CP053745:409950-410216 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPASTSDEPRYLVIGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T157688 NZ_CP069447:562007-562114 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT157688 WP_000643598.1 NZ_CP053745:410203-410448 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT157688 NZ_CP069447:c561958-561895 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Antitoxin

• AlphaFold DB: A0A271VME2