Detailed information of TA system TA157686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-phd/HigB(toxin)
• Location: 402851..403301
• Replicon: chromosome
• Accession: NZ_CP053745
• Organism: Vibrio cholerae strain SA3G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY04_RS15625
• Protein ID: WP_001162670.1
• Coordinates: 403014..403301 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HPY04_RS15620
• Protein ID: WP_175249423.1
• Coordinates: 402851..402973 (+)
• Length: 41 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY04_RS15585	397876..398301	+	426	WP_071171304.1	hypothetical protein	-
HPY04_RS15590	398571..398897	+	327	WP_175249420.1	hypothetical protein	-
HPY04_RS15595	399112..399357	-	246	WP_175249421.1	hypothetical protein	-
HPY04_RS15600	399916..400263	+	348	WP_001071511.1	VOC family protein	-
HPY04_RS15605	400383..401045	+	663	WP_000084911.1	Vat family streptogramin A O-acetyltransferase	-
HPY04_RS15610	401341..401523	+	183	WP_000923154.1	DUF645 family protein	-
HPY04_RS15615	402222..402782	+	561	WP_175249422.1	nucleotidyltransferase domain-containing protein	-
HPY04_RS15620	402851..402973	+	123	WP_175249423.1	acetyltransferase	Antitoxin
HPY04_RS15625	403014..403301	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY04_RS15630	403312..403629	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
HPY04_RS15635	403773..404312	+	540	WP_175231429.1	hydrolase	-
HPY04_RS15640	404463..404888	+	426	WP_095462070.1	GNAT family N-acetyltransferase	-
HPY04_RS15645	405238..405333	+	96	WP_175249424.1	DUF645 family protein	-
HPY04_RS15650	405543..406001	+	459	WP_032473158.1	GNAT family N-acetyltransferase	-
HPY04_RS15655	406318..406497	+	180	WP_050547145.1	DUF645 family protein	-
HPY04_RS15660	406646..406807	+	162	Protein_398	DUF3709 domain-containing protein	-
HPY04_RS15665	406843..406995	+	153	WP_175242275.1	DUF3709 domain-containing protein	-
HPY04_RS15670	407459..407668	+	210	WP_175249665.1	DUF645 family protein	-
HPY04_RS15675	407879..408262	+	384	WP_175249425.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	336942..428561	91619
	-	inside	Integron	-	-	388738..428561	39823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T157686 WP_001162670.1 NZ_CP053745:403014-403301 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T157686 NZ_CP069447:c128863-128552 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 41 a.a.
• Molecular weight: 4604.75 Da
• Isoelectric Point: 12.1021

>AT157686 WP_175249423.1 NZ_CP053745:402851-402973 [Vibrio cholerae]
VVSVVVLKFSGMRCQPLRRALCTFLKSFLQIKLVHSVIMR

Nucleotide

• Length: 123 bp

>AT157686 NZ_CP069447:c128551-128261 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0F2HI36