Detailed information of TA system TA157682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 347934..348499
• Replicon: chromosome
• Accession: NZ_CP053745
• Organism: Vibrio cholerae strain SA3G

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D7HCW8
• Locus tag: HPY04_RS15180
• Protein ID: WP_000869999.1
• Coordinates: 348212..348499 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D0IIK3
• Locus tag: HPY04_RS15175
• Protein ID: WP_000086649.1
• Coordinates: 347934..348215 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY04_RS15135	343081..343875	+	795	WP_175249392.1	hypothetical protein	-
HPY04_RS15140	343893..343982	+	90	WP_072036367.1	DUF3265 domain-containing protein	-
HPY04_RS15145	344017..344514	+	498	WP_000259910.1	GNAT family N-acetyltransferase	-
HPY04_RS15150	344802..344984	+	183	WP_000923184.1	DUF645 family protein	-
HPY04_RS15155	345201..345938	+	738	WP_175249393.1	SDR family oxidoreductase	-
HPY04_RS15160	346036..346407	+	372	WP_001891306.1	DUF4144 domain-containing protein	-
HPY04_RS15165	346555..347067	+	513	WP_069730860.1	hypothetical protein	-
HPY04_RS15170	347210..347725	+	516	WP_069217449.1	lipocalin family protein	-
HPY04_RS15175	347934..348215	+	282	WP_000086649.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HPY04_RS15180	348212..348499	+	288	WP_000869999.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY04_RS15185	348650..349180	+	531	WP_055034297.1	hypothetical protein	-
HPY04_RS15190	349482..349568	+	87	WP_134820423.1	DUF645 family protein	-
HPY04_RS15195	349571..349660	+	90	Protein_305	DUF645 family protein	-
HPY04_RS15200	349858..350604	+	747	WP_033929253.1	HNH endonuclease	-
HPY04_RS15205	350968..351146	+	179	Protein_307	DUF645 family protein	-
HPY04_RS15210	351337..351825	+	489	WP_175249394.1	GNAT family N-acetyltransferase	-
HPY04_RS15215	351972..352724	+	753	WP_175249395.1	hypothetical protein	-
HPY04_RS15220	352786..352848	+	63	Protein_310	acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	336942..428561	91619
	-	inside	Integron	-	-	338106..368673	30567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10997.74 Da
• Isoelectric Point: 6.0710

>T157682 WP_000869999.1 NZ_CP053745:348212-348499 [Vibrio cholerae]
MKVVWSPLALQKLGDAAEFIALDNPSAAEKWVNEVFDKTELLGSMPEMGRMVPEMPHTNYREIIFGHYRIIYSLSHEIRV
LTVRNCRQLLTEHDV

Nucleotide

• Length: 288 bp

>T157682 NZ_CP069445:4396626-4396940 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10361.93 Da
• Isoelectric Point: 5.1548

>AT157682 WP_000086649.1 NZ_CP053745:347934-348215 [Vibrio cholerae]
MSRIHLDQDIQPLSEFRAGVASFIKQINETRRPLVITQRGKGVAVVLDVAEYEAMQEKIELLEEMRTAEAQLAAGLGISN
EDARSQVLGRIIK

Nucleotide

• Length: 282 bp

>AT157682 NZ_CP069445:4396390-4396623 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A0H3AEF1

Antitoxin

• AlphaFold DB: A0A0K9UIW4