Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 8544..9066 Replicon plasmid pCP8-3-IncX1
Accession NZ_CP053740
Organism Escherichia coli strain CP8-3_Sichuan

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag HP430_RS24020 Protein ID WP_000220560.1
Coordinates 8544..8825 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HP430_RS24025 Protein ID WP_000121743.1
Coordinates 8815..9066 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP430_RS23975 4392..5558 - 1167 WP_000539530.1 relaxase/mobilization nuclease domain-containing protein -
HP430_RS23980 5561..6106 - 546 WP_015060502.1 hypothetical protein -
HP430_RS23985 6432..6704 - 273 WP_000160399.1 hypothetical protein -
HP430_RS23990 6717..6866 - 150 WP_000003880.1 hypothetical protein -
HP430_RS23995 6936..7115 - 180 WP_000439078.1 hypothetical protein -
HP430_RS24000 7158..7487 - 330 WP_000866648.1 hypothetical protein -
HP430_RS24005 7580..7795 - 216 WP_001180116.1 hypothetical protein -
HP430_RS24010 7785..8030 - 246 WP_000356546.1 hypothetical protein -
HP430_RS24015 8075..8398 - 324 WP_001181903.1 hypothetical protein -
HP430_RS24020 8544..8825 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HP430_RS24025 8815..9066 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HP430_RS24030 10300..11136 + 837 WP_001050931.1 replication initiation protein -
HP430_RS24035 11176..11622 + 447 WP_001074386.1 hypothetical protein -
HP430_RS24040 11759..12112 + 354 WP_000876434.1 hypothetical protein -
HP430_RS24045 12172..13140 - 969 WP_031942372.1 IS5 family transposase -
HP430_RS24050 13142..13435 - 294 WP_015060510.1 hypothetical protein -
HP430_RS24055 13451..13681 - 231 WP_000051064.1 partitioning protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkF / mrkD / mrkC / mrkB / mrkA 1..38002 38002


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T157680 WP_000220560.1 NZ_CP053740:c8825-8544 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T157680 NZ_CP053740:c8825-8544 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT157680 WP_000121743.1 NZ_CP053740:c9066-8815 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT157680 NZ_CP053740:c9066-8815 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References