Detailed information of TA system TA157679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 27796..28439
• Replicon: plasmid pCP8-3-IncR
• Accession: NZ_CP053739
• Organism: Escherichia coli strain CP8-3_Sichuan

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HP430_RS23855
• Protein ID: WP_062934173.1
• Coordinates: 28023..28439 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HP430_RS23850
• Protein ID: WP_001261276.1
• Coordinates: 27796..28026 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP430_RS23820	23356..24222	-	867	WP_004118283.1	replication initiation protein	-
HP430_RS23825	24757..24861	+	105	WP_032409716.1	hypothetical protein	-
HP430_RS23830	24990..25247	+	258	WP_000764642.1	hypothetical protein	-
HP430_RS23835	25305..26081	-	777	WP_000015958.1	site-specific integrase	-
HP430_RS23840	26078..26821	-	744	WP_000129823.1	hypothetical protein	-
HP430_RS23845	26872..27222	-	351	WP_004118279.1	hypothetical protein	-
HP430_RS23850	27796..28026	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HP430_RS23855	28023..28439	+	417	WP_062934173.1	type II toxin-antitoxin system VapC family toxin	Toxin
HP430_RS23860	28643..29328	+	686	Protein_28	IS3-like element ISEc15 family transposase	-
HP430_RS23865	29997..31505	+	1509	WP_172614375.1	group II intron reverse transcriptase/maturase	-
HP430_RS23870	31600..31716	+	117	Protein_30	IS200/IS605 family transposase	-
HP430_RS23875	31900..33180	-	1281	WP_088475062.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-3.5 / qnrS1 / blaTEM-1B / tet(M)	-	1..47220	47220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15050.57 Da
• Isoelectric Point: 9.2957

>T157679 WP_062934173.1 NZ_CP053739:28023-28439 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T157679 NZ_CP069445:c4277966-4277628 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCTCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCAAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGAGTTTATTGGGGTGATTAG
TAGCAAAACGCCACGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT157679 WP_001261276.1 NZ_CP053739:27796-28026 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT157679 NZ_CP069445:4277984-4278037 [Escherichia coli]
AATAGGGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: A0A387K023