Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 9129..9594 Replicon plasmid pCP55-IncX4
Accession NZ_CP053735
Organism Escherichia coli strain CP55_Sichuan

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag HP431_RS26340 Protein ID WP_001230707.1
Coordinates 9343..9594 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag HP431_RS26335 Protein ID WP_000801440.1
Coordinates 9129..9356 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP431_RS26295 4359..4574 - 216 WP_000760375.1 EexN family lipoprotein -
HP431_RS26300 4571..4864 - 294 WP_060598542.1 LuxR family transcriptional regulator -
HP431_RS26305 4941..6170 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
HP431_RS26310 6174..6677 - 504 WP_000781813.1 hypothetical protein -
HP431_RS26315 7038..7385 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
HP431_RS26320 7382..7630 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
HP431_RS26325 7935..8285 - 351 WP_000854259.1 hypothetical protein -
HP431_RS26330 8376..8675 - 300 WP_000650309.1 hypothetical protein -
HP431_RS26335 9129..9356 - 228 WP_000801440.1 hypothetical protein Antitoxin
HP431_RS26340 9343..9594 - 252 WP_001230707.1 hypothetical protein Toxin
HP431_RS26345 9591..10133 - 543 WP_001083906.1 hypothetical protein -
HP431_RS26350 10238..10753 - 516 WP_001025395.1 J domain-containing protein -
HP431_RS26355 12168..13034 + 867 WP_001353743.1 replication initiation protein -
HP431_RS26360 13047..13451 + 405 WP_012421203.1 hypothetical protein -
HP431_RS26365 13586..14113 + 528 WP_000577043.1 DUF2726 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33309 33309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T157653 WP_001230707.1 NZ_CP053735:c9594-9343 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T157653 NZ_CP069441:c3487721-3487383 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAACCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAACAAAACGCCACGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT157653 WP_000801440.1 NZ_CP053735:c9356-9129 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT157653 NZ_CP069441:3487716-3487792 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References