Detailed information of TA system TA157652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 7038..7630
• Replicon: plasmid pCP55-IncX4
• Accession: NZ_CP053735
• Organism: Escherichia coli strain CP55_Sichuan

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: HP431_RS26320
• Protein ID: WP_000520549.1
• Coordinates: 7382..7630 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: HP431_RS26315
• Protein ID: WP_000681613.1
• Coordinates: 7038..7385 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP431_RS26285	3043..3315	-	273	WP_001328551.1	TrbC/VirB2 family protein	-
HP431_RS26290	3383..4003	-	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
HP431_RS26295	4359..4574	-	216	WP_000760375.1	EexN family lipoprotein	-
HP431_RS26300	4571..4864	-	294	WP_060598542.1	LuxR family transcriptional regulator	-
HP431_RS26305	4941..6170	-	1230	WP_001329504.1	relaxase/mobilization nuclease domain-containing protein	-
HP431_RS26310	6174..6677	-	504	WP_000781813.1	hypothetical protein	-
HP431_RS26315	7038..7385	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HP431_RS26320	7382..7630	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
HP431_RS26325	7935..8285	-	351	WP_000854259.1	hypothetical protein	-
HP431_RS26330	8376..8675	-	300	WP_000650309.1	hypothetical protein	-
HP431_RS26335	9129..9356	-	228	WP_000801440.1	hypothetical protein	-
HP431_RS26340	9343..9594	-	252	WP_001230707.1	hypothetical protein	-
HP431_RS26345	9591..10133	-	543	WP_001083906.1	hypothetical protein	-
HP431_RS26350	10238..10753	-	516	WP_001025395.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33309	33309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T157652 WP_000520549.1 NZ_CP053735:c7630-7382 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T157652 NZ_CP069441:c3453201-3452944 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT157652 WP_000681613.1 NZ_CP053735:c7385-7038 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT157652 NZ_CP069441:c3452932-3452381 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1