Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 34366..34891 Replicon plasmid pCP55-141k
Accession NZ_CP053733
Organism Escherichia coli strain CP55_Sichuan

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HP431_RS25215 Protein ID WP_001159871.1
Coordinates 34586..34891 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag HP431_RS25210 Protein ID WP_000813630.1
Coordinates 34366..34584 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP431_RS25190 29472..30341 - 870 WP_000253407.1 hypothetical protein -
HP431_RS25195 30343..31431 - 1089 WP_000952231.1 hypothetical protein -
HP431_RS25200 31729..32661 - 933 WP_000991830.1 hypothetical protein -
HP431_RS25205 32665..33660 - 996 WP_000246635.1 hypothetical protein -
HP431_RS25210 34366..34584 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HP431_RS25215 34586..34891 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HP431_RS25220 34892..35698 + 807 WP_077255603.1 site-specific integrase -
HP431_RS25225 35879..36523 + 645 WP_001144036.1 ParA family protein -
HP431_RS25230 36610..36918 + 309 WP_000030199.1 hypothetical protein -
HP431_RS25235 37332..38312 + 981 WP_032331146.1 plasmid segregation protein ParM -
HP431_RS25240 38305..38721 + 417 WP_001278818.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP 1..141846 141846


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T157647 WP_001159871.1 NZ_CP053733:34586-34891 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T157647 NZ_CP069441:c1551253-1550846 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT157647 WP_000813630.1 NZ_CP053733:34366-34584 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT157647 NZ_CP069441:c1551500-1551234 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References