Detailed information of TA system TA157646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 1833..2368
• Replicon: plasmid pCP55-141k
• Accession: NZ_CP053733
• Organism: Escherichia coli strain CP55_Sichuan

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HP431_RS25055
• Protein ID: WP_032331135.1
• Coordinates: 2081..2368 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A5N8HB59
• Locus tag: HP431_RS25050
• Protein ID: WP_032331134.1
• Coordinates: 1833..2084 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP431_RS25045	1..873	+	873	WP_001536607.1	incFII family plasmid replication initiator RepA	-
HP431_RS25050	1833..2084	+	252	WP_032331134.1	antitoxin	Antitoxin
HP431_RS25055	2081..2368	+	288	WP_032331135.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HP431_RS25060	2472..3075	+	604	Protein_3	serine acetyltransferase	-
HP431_RS25065	3267..3964	+	698	Protein_4	IS1 family transposase	-
HP431_RS25070	4220..5062	+	843	WP_170969611.1	polysaccharide deacetylase family protein	-
HP431_RS25075	5065..6153	+	1089	WP_072041713.1	putative hexosyltransferase CapU	-
HP431_RS25080	6158..7108	+	951	WP_072041714.1	virulence factor VirK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP	1..141846	141846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11133.16 Da
• Isoelectric Point: 10.6261

>T157646 WP_032331135.1 NZ_CP053733:2081-2368 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T157646 NZ_CP069441:c1320561-1320250 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACACCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9243.47 Da
• Isoelectric Point: 4.0927

>AT157646 WP_032331134.1 NZ_CP053733:1833-2084 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT157646 NZ_CP069441:c1320253-1319834 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A5N8HB59