Detailed information of TA system TA157641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 31787..32334
• Replicon: plasmid pCP55-IncFIB
• Accession: NZ_CP053732
• Organism: Escherichia coli strain CP55_Sichuan

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HP431_RS24315
• Protein ID: WP_000969990.1
• Coordinates: 31787..32068 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: HP431_RS24320
• Protein ID: WP_000079941.1
• Coordinates: 32065..32334 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP431_RS24295	28052..29086	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
HP431_RS24300	29645..29992	-	348	WP_000142437.1	hypothetical protein	-
HP431_RS24305	30294..30776	-	483	WP_001311056.1	hypothetical protein	-
HP431_RS24310	30893..31741	-	849	WP_001058005.1	3'-5' exonuclease	-
HP431_RS24315	31787..32068	-	282	WP_000969990.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HP431_RS24320	32065..32334	-	270	WP_000079941.1	hypothetical protein	Antitoxin
HP431_RS24325	32856..34092	+	1237	Protein_26	IS3 family transposase	-
HP431_RS24330	34289..35563	+	1275	WP_000489607.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN	1..156025	156025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10930.61 Da
• Isoelectric Point: 8.4615

>T157641 WP_000969990.1 NZ_CP053732:c32068-31787 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTVQKWP

Nucleotide

• Length: 282 bp

>T157641 NZ_CP069439:149023-149310 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAAATGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGCGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT157641 WP_000079941.1 NZ_CP053732:c32334-32065 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT157641 NZ_CP069439:148775-149026 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5