Detailed information of TA system TA157638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4308371..4308996
• Replicon: chromosome
• Accession: NZ_CP053731
• Organism: Escherichia coli strain CP55_Sichuan

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HP431_RS20775
• Protein ID: WP_000911330.1
• Coordinates: 4308598..4308996 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HP431_RS20770
• Protein ID: WP_000450524.1
• Coordinates: 4308371..4308598 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP431_RS20745	4304174..4304644	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HP431_RS20750	4304644..4305216	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HP431_RS20755	4305362..4306240	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HP431_RS20760	4306257..4307291	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HP431_RS20765	4307504..4308217	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HP431_RS20770	4308371..4308598	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HP431_RS20775	4308598..4308996	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HP431_RS20780	4309143..4310006	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
HP431_RS20785	4310021..4312036	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
HP431_RS20790	4312110..4312808	+	699	WP_000679823.1	esterase	-
HP431_RS20795	4312918..4313118	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T157638 WP_000911330.1 NZ_CP053731:4308598-4308996 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T157638 NZ_CP069438:4910427-4910801 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATATGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT157638 WP_000450524.1 NZ_CP053731:4308371-4308598 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT157638 NZ_CP069438:4910006-4910380 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33