Detailed information of TA system TA157637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3956665..3957248
• Replicon: chromosome
• Accession: NZ_CP053731
• Organism: Escherichia coli strain CP55_Sichuan

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U9XFN8
• Locus tag: HP431_RS18945
• Protein ID: WP_000254745.1
• Coordinates: 3956913..3957248 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HP431_RS18940
• Protein ID: WP_113440871.1
• Coordinates: 3956665..3956913 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP431_RS18930	3953004..3954305	+	1302	WP_000046790.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
HP431_RS18935	3954353..3956587	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
HP431_RS18940	3956665..3956913	+	249	WP_113440871.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HP431_RS18945	3956913..3957248	+	336	WP_000254745.1	endoribonuclease MazF	Toxin
HP431_RS18950	3957319..3958110	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
HP431_RS18955	3958338..3959975	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HP431_RS18960	3960063..3961361	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
HP431_RS18965	3961417..3961779	-	363	WP_000034929.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12158.08 Da
• Isoelectric Point: 8.4777

>T157637 WP_000254745.1 NZ_CP053731:3956913-3957248 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T157637 NZ_CP069438:c4297802-4297584 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9387.80 Da
• Isoelectric Point: 4.4665

>AT157637 WP_113440871.1 NZ_CP053731:3956665-3956913 [Escherichia coli]
MIHSSVKRWGNSPAMRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT157637 NZ_CP069438:c4298202-4297828 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0XYM3