Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3820331..3820985 Replicon chromosome
Accession NZ_CP053731
Organism Escherichia coli strain CP55_Sichuan

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HP431_RS18330 Protein ID WP_000244772.1
Coordinates 3820578..3820985 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HP431_RS18325 Protein ID WP_000354046.1
Coordinates 3820331..3820597 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP431_RS18300 3815619..3816362 + 744 WP_000951964.1 SDR family oxidoreductase -
HP431_RS18305 3816419..3817852 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
HP431_RS18310 3817897..3818208 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
HP431_RS18315 3818372..3819031 + 660 WP_000250274.1 hemolysin III family protein -
HP431_RS18320 3819108..3820088 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HP431_RS18325 3820331..3820597 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HP431_RS18330 3820578..3820985 + 408 WP_000244772.1 protein YgfX Toxin
HP431_RS18335 3821025..3821546 - 522 WP_001055874.1 flavodoxin FldB -
HP431_RS18340 3821658..3822554 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HP431_RS18345 3822579..3823289 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HP431_RS18350 3823295..3825028 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T157636 WP_000244772.1 NZ_CP053731:3820578-3820985 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T157636 NZ_CP069438:3837750-3838064 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT157636 WP_000354046.1 NZ_CP053731:3820331-3820597 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT157636 NZ_CP069438:3837514-3837747 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References