Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3189401..3190201 Replicon chromosome
Accession NZ_CP053731
Organism Escherichia coli strain CP55_Sichuan

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag HP431_RS15185 Protein ID WP_000342449.1
Coordinates 3189674..3190201 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag HP431_RS15180 Protein ID WP_001277108.1
Coordinates 3189401..3189667 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP431_RS15160 3185059..3185727 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
HP431_RS15165 3185720..3186778 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
HP431_RS15170 3187023..3187877 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HP431_RS15175 3188148..3189251 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HP431_RS15180 3189401..3189667 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
HP431_RS15185 3189674..3190201 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
HP431_RS15190 3190198..3190581 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
HP431_RS15195 3191005..3192114 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HP431_RS15200 3192162..3193088 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HP431_RS15205 3193085..3194362 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
HP431_RS15210 3194359..3195126 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T157634 WP_000342449.1 NZ_CP053731:3189674-3190201 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T157634 NZ_CP069438:3731789-3731998 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT157634 WP_001277108.1 NZ_CP053731:3189401-3189667 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT157634 NZ_CP069438:3731995-3732297 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References