Detailed information of TA system TA157629

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 2164801..2165504
• Replicon: chromosome
• Accession: NZ_CP053731
• Organism: Escherichia coli strain CP55_Sichuan

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A853JWF2
• Locus tag: HP431_RS10460
• Protein ID: WP_032267594.1
• Coordinates: 2164801..2165142 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HP431_RS10465
• Protein ID: WP_032267593.1
• Coordinates: 2165163..2165504 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP431_RS10440	2159912..2160892	+	981	WP_000991444.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HP431_RS10445	2161104..2161976	+	873	WP_000168567.1	HNH endonuclease	-
HP431_RS10450	2162087..2163085	-	999	WP_001240355.1	membrane protein	-
HP431_RS10455	2163543..2164550	-	1008	WP_032267595.1	restriction endonuclease	-
HP431_RS10460	2164801..2165142	-	342	WP_032267594.1	TA system toxin CbtA family protein	Toxin
HP431_RS10465	2165163..2165504	-	342	WP_032267593.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HP431_RS10470	2165515..2166057	-	543	WP_172615514.1	DNA repair protein RadC	-
HP431_RS10475	2166070..2166513	-	444	WP_032267591.1	antirestriction protein	-
HP431_RS10480	2166544..2167365	-	822	WP_032267590.1	DUF945 domain-containing protein	-
HP431_RS10485	2167486..2167959	-	474	WP_032279719.1	hypothetical protein	-
HP431_RS10490	2168054..2168594	-	541	Protein_2058	DUF4339 domain-containing protein	-
HP431_RS10495	2168661..2169121	-	461	Protein_2059	hypothetical protein	-
HP431_RS10500	2169118..2169573	-	456	WP_057688286.1	hypothetical protein	-
HP431_RS10505	2169612..2170247	-	636	WP_032267586.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	2153242..2187837	34595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (13-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12767.68 Da
• Isoelectric Point: 6.8699

>T157629 WP_032267594.1 NZ_CP053731:c2165142-2164801 [Escherichia coli]
MKTLPATTPQAATLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDEKVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
GWQEQSPYLRAVDILRARQATGLLKARHGEHWE

Nucleotide

• Length: 342 bp

>T157629 NZ_CP069438:2846365-2846742 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12760.46 Da
• Isoelectric Point: 6.6213

>AT157629 WP_032267593.1 NZ_CP053731:c2165504-2165163 [Escherichia coli]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDLAFPHFISQMESMLTTGEINPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYLTYR

Nucleotide

• Length: 342 bp

>AT157629 NZ_CP069438:2845907-2846275 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A853JWF2