Detailed information of TA system TA157602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3078326..3078852
• Replicon: chromosome
• Accession: NZ_CP053730
• Organism: Escherichia coli strain CP61_Sichuan

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HP432_RS15760
• Protein ID: WP_000323025.1
• Coordinates: 3078326..3078613 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: HP432_RS15765
• Protein ID: WP_000534858.1
• Coordinates: 3078613..3078852 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP432_RS15730	3073630..3074433	-	804	Protein_2880	type VI secretion system tip protein VgrG	-
HP432_RS15735	3075253..3075465	-	213	WP_000226995.1	YncH family protein	-
HP432_RS15740	3075541..3076158	-	618	WP_000598851.1	glutathione S-transferase family protein	-
HP432_RS15750	3077631..3077882	-	252	WP_001463433.1	hypothetical protein	-
HP432_RS15755	3078099..3078254	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
HP432_RS15760	3078326..3078613	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HP432_RS15765	3078613..3078852	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HP432_RS15770	3078877..3079182	+	306	WP_071593935.1	hypothetical protein	-
HP432_RS15775	3079385..3079717	+	333	WP_001301033.1	protein FlxA	-
HP432_RS15780	3080154..3080303	-	150	WP_011443592.1	hypothetical protein	-
HP432_RS15785	3080341..3081417	-	1077	Protein_2891	starvation-sensing protein RspA	-
HP432_RS15790	3081623..3081949	-	327	WP_001469615.1	YnfA family protein	-
HP432_RS15795	3082084..3082425	+	342	WP_000705197.1	DUF1283 family protein	-
HP432_RS15800	3082460..3083020	+	561	WP_001138582.1	spermidine N1-acetyltransferase	-
HP432_RS15805	3083023..3083733	-	711	WP_072104773.1	YnfC family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	vgrG/tssI	3066697..3084146	17449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T157602 WP_000323025.1 NZ_CP053730:c3078613-3078326 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T157602 NZ_CP069386:c3669416-3669138 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT157602 WP_000534858.1 NZ_CP053730:c3078852-3078613 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT157602 NZ_CP069386:c3669679-3669419 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8