Detailed information of TA system TA157594

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1718563..1719242
• Replicon: chromosome
• Accession: NZ_CP053730
• Organism: Escherichia coli strain CP61_Sichuan

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: HP432_RS09090
• Protein ID: WP_000854680.1
• Coordinates: 1718563..1718904 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: HP432_RS09095
• Protein ID: WP_000070396.1
• Coordinates: 1718925..1719242 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP432_RS09065	1713840..1714241	+	402	WP_000174677.1	sigma factor-binding protein Crl	-
HP432_RS09070	1714280..1715335	-	1056	WP_000749863.1	phosphoporin PhoE	-
HP432_RS09075	1715623..1716726	+	1104	WP_001285288.1	glutamate 5-kinase	-
HP432_RS09080	1716738..1717991	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
HP432_RS09090	1718563..1718904	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HP432_RS09095	1718925..1719242	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HP432_RS09100	1719261..1719482	-	222	WP_000691994.1	DUF987 domain-containing protein	-
HP432_RS09105	1719491..1719967	-	477	WP_000811693.1	RadC family protein	-
HP432_RS09110	1719983..1720441	-	459	WP_000211838.1	antirestriction protein	-
HP432_RS09115	1720539..1720778	-	240	Protein_1581	DUF905 domain-containing protein	-
HP432_RS09120	1720855..1721322	-	468	WP_001385283.1	hypothetical protein	-
HP432_RS09125	1721345..1721788	-	444	WP_000649865.1	hypothetical protein	-
HP432_RS09130	1721788..1722024	-	237	WP_001144031.1	hypothetical protein	-
HP432_RS09135	1722065..1722766	-	702	WP_047649085.1	WYL domain-containing protein	-
HP432_RS09140	1722983..1723804	-	822	WP_023565123.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1708384..1739490	31106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T157594 WP_000854680.1 NZ_CP053730:c1718904-1718563 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T157594 NZ_CP069386:c1859234-1858920 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT157594 WP_000070396.1 NZ_CP053730:c1719242-1718925 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT157594 NZ_CP069386:c1858923-1858507 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7