Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 67675..68197 Replicon plasmid pCP61-IncN
Accession NZ_CP053728
Organism Escherichia coli strain CP61_Sichuan

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag HP432_RS00375 Protein ID WP_000220560.1
Coordinates 67675..67956 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HP432_RS00380 Protein ID WP_000121743.1
Coordinates 67946..68197 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP432_RS00335 63240..63896 - 657 WP_012537714.1 quinolone resistance pentapeptide repeat protein QnrS2 -
HP432_RS00340 64195..64470 - 276 Protein_67 IS30 family transposase -
HP432_RS00345 64536..65240 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
HP432_RS00350 65302..65499 - 198 WP_000844102.1 hypothetical protein -
HP432_RS00355 65504..66145 - 642 WP_001694850.1 restriction endonuclease -
HP432_RS00360 66201..66512 - 312 WP_001452736.1 hypothetical protein -
HP432_RS00365 66548..66862 - 315 WP_000734776.1 IncN plasmid KikA protein -
HP432_RS00370 66903..67600 - 698 Protein_73 IS1 family transposase -
HP432_RS00375 67675..67956 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HP432_RS00380 67946..68197 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HP432_RS00385 69409..70245 + 837 WP_001050931.1 replication initiation protein -
HP432_RS00390 70285..70731 + 447 Protein_77 hypothetical protein -
HP432_RS00395 70868..71224 + 357 WP_000456533.1 hypothetical protein -
HP432_RS00400 71221..72198 - 978 WP_024245156.1 hypothetical protein -
HP432_RS00405 72223..72504 - 282 WP_001185482.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / aph(3')-Ia / qnrS2 - 1..80920 80920


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T157588 WP_000220560.1 NZ_CP053728:c67956-67675 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T157588 NZ_CP069386:c720029-719778 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT157588 WP_000121743.1 NZ_CP053728:c68197-67946 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT157588 NZ_CP069386:c719781-719527 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References