Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 16971..17614 Replicon plasmid pCP61-IncN
Accession NZ_CP053728
Organism Escherichia coli strain CP61_Sichuan

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HP432_RS00110 Protein ID WP_000754566.1
Coordinates 17198..17614 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HP432_RS00105 Protein ID WP_001261276.1
Coordinates 16971..17201 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP432_RS00075 12531..13397 - 867 WP_004118283.1 replication initiation protein -
HP432_RS00080 13932..14036 + 105 WP_032409716.1 hypothetical protein -
HP432_RS00085 14165..14422 + 258 WP_000764642.1 hypothetical protein -
HP432_RS00090 14480..15256 - 777 WP_000015958.1 site-specific integrase -
HP432_RS00095 15253..15996 - 744 WP_000129823.1 hypothetical protein -
HP432_RS00100 16047..16397 - 351 WP_004118279.1 hypothetical protein -
HP432_RS00105 16971..17201 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HP432_RS00110 17198..17614 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HP432_RS00115 17818..18503 + 686 Protein_22 IS3-like element ISEc15 family transposase -
HP432_RS00120 19172..20680 + 1509 WP_172614375.1 group II intron reverse transcriptase/maturase -
HP432_RS00125 20775..20891 + 117 Protein_24 IS200/IS605 family transposase -
HP432_RS00130 21075..22355 - 1281 WP_088475062.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / aph(3')-Ia / qnrS2 - 1..80920 80920
- flank IS/Tn - - 17818..18102 284


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T157587 WP_000754566.1 NZ_CP053728:17198-17614 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T157587 NZ_CP069386:c719781-719527 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT157587 WP_001261276.1 NZ_CP053728:16971-17201 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT157587 NZ_CP069386:c720029-719778 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References