Detailed information of TA system TA157573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 56031..56566
• Replicon: plasmid pCP66-6-IncFIC
• Accession: NZ_CP053724
• Organism: Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: HP433_RS23415
• Protein ID: WP_000222766.1
• Coordinates: 56031..56318 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: HP433_RS23420
• Protein ID: WP_001132900.1
• Coordinates: 56315..56566 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP433_RS23385	51400..52260	-	861	WP_000557454.1	aminoglycoside N-acetyltransferase AAC(3)-IId	-
HP433_RS23390	52403..53077	+	675	Protein_61	IS4-like element ISVsa5 family transposase	-
HP433_RS23395	53130..53834	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
HP433_RS23400	53902..55200	+	1299	Protein_63	Tn3-like element Tn3 family transposase	-
HP433_RS23405	55234..55734	+	501	Protein_64	Tn3 family transposase	-
HP433_RS23410	55768..55956	+	189	Protein_65	transposase	-
HP433_RS23415	56031..56318	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HP433_RS23420	56315..56566	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HP433_RS23425	57532..58389	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
HP433_RS23430	58382..58456	-	75	WP_061357229.1	RepA leader peptide Tap	-
HP433_RS23435	58688..58945	-	258	WP_001541895.1	replication regulatory protein RepA	-
HP433_RS23440	59213..59419	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
-	59406..59467	+	62	NuclAT_1	-	-
-	59406..59467	+	62	NuclAT_1	-	-
-	59406..59467	+	62	NuclAT_1	-	-
-	59406..59467	+	62	NuclAT_1	-	-
HP433_RS23445	59809..60021	-	213	WP_001541896.1	ANR family transcriptional regulator	-
HP433_RS23450	60156..60566	-	411	Protein_73	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / aph(3')-Ia / sul3 / blaTEM-215 / lnu(F) / aac(3)-IId	-	1..99734	99734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T157573 WP_000222766.1 NZ_CP053724:c56318-56031 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T157573 NZ_CP069381:2342298-2342582 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT157573 WP_001132900.1 NZ_CP053724:c56566-56315 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT157573 NZ_CP069381:2342060-2342308 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2

Antitoxin

• AlphaFold DB: V0VIP2