Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4339164..4339661 Replicon chromosome
Accession NZ_CP053723
Organism Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HP433_RS20910 Protein ID WP_001173343.1
Coordinates 4339164..4339337 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HP433_RS20915 Protein ID WP_000398619.1
Coordinates 4339365..4339661 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP433_RS20900 4335834..4337291 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HP433_RS20905 4337528..4339045 + 1518 WP_001295074.1 lysine--tRNA ligase -
HP433_RS20910 4339164..4339337 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HP433_RS20915 4339365..4339661 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HP433_RS20920 4339888..4340160 - 273 WP_000405649.1 N-acetyltransferase -
HP433_RS20925 4340172..4340402 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
HP433_RS20930 4340583..4342214 + 1632 WP_001216487.1 sensor histidine kinase -
HP433_RS20935 4342211..4342930 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T157571 WP_001173343.1 NZ_CP053723:c4339337-4339164 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T157571 NZ_CP069381:c987900-987373 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT157571 WP_000398619.1 NZ_CP053723:c4339661-4339365 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT157571 NZ_CP069381:c988187-987897 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References