157568

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3606903..3607740 Replicon chromosome
Accession NZ_CP053723
Organism Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HP433_RS17420 Protein ID WP_000227784.1
Coordinates 3607198..3607740 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HP433_RS17415 Protein ID WP_001297137.1
Coordinates 3606903..3607214 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP433_RS17390 3601923..3602870 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
HP433_RS17395 3602892..3604883 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HP433_RS17400 3604873..3605487 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HP433_RS17405 3605487..3605816 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HP433_RS17410 3605828..3606718 + 891 WP_000971336.1 heme o synthase -
HP433_RS17415 3606903..3607214 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HP433_RS17420 3607198..3607740 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HP433_RS17425 3607796..3608731 - 936 WP_001365761.1 sel1 repeat family protein -
HP433_RS17430 3609139..3610503 + 1365 WP_001000974.1 MFS transporter -
HP433_RS17435 3610631..3611122 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HP433_RS17440 3611290..3612201 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T157568 WP_000227784.1 NZ_CP053723:3607198-3607740 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T157568 NZ_CP069381:359208-359483 [Salmonella enterica]
ATGGGGCAAAGGAAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGGGCCTGACTGGATTCTTATTGATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT157568 WP_001297137.1 NZ_CP053723:3606903-3607214 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT157568 NZ_CP069381:358945-359205 [Salmonella enterica]
ATGGCTGCAAATGTGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGAATCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGGATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGACATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References