Detailed information of TA system TA157565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2484472..2486060
• Replicon: chromosome
• Accession: NZ_CP053723
• Organism: Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: E0IX85
• Locus tag: HP433_RS12090
• Protein ID: WP_001125454.1
• Coordinates: 2484738..2486060 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HP433_RS12085
• Protein ID: WP_001296726.1
• Coordinates: 2484472..2484738 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP433_RS12080	2480370..2484263	+	3894	Protein_2363	autotransporter barrel domain-containing lipoprotein	-
HP433_RS12085	2484472..2484738	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HP433_RS12090	2484738..2486060	+	1323	WP_001125454.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HP433_RS12095	2486372..2486580	+	209	Protein_2366	helix-turn-helix transcriptional regulator	-
HP433_RS12100	2487012..2487575	+	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
HP433_RS12105	2487936..2488646	+	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2484472..2503282	18810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49307.90 Da
• Isoelectric Point: 8.8753

>T157565 WP_001125454.1 NZ_CP053723:2484738-2486060 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T157565 NZ_CP069372:36798-37193 [Neotabrizicola shimadae]
ATGAGCGCTGAATTCGCGGACACGAATGTCGTTCTGTACCTGCTCGACGACGGCCCGAAGGCCGATCGCGCCGAGCTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAGGTGCTGAACGAGACGCTGGTAAACTGCCGCCGCAAAGCCGGCCTCAGTT
GGGAGGAGGCAGGGACGTTTCTCGAAGGCGTGCGCGCCTTGTGCCCCGTCGAAGACCTGACCCTCCAAACCCATGACGTT
GGCCGCGCCTTGGCGGAACGTTACGGTTTCTCGATCTACGACGCGATGATCGTGGCAAGCGCTCTGGTTGCGGGCTGCAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTAGTCGAAGGGCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT157565 WP_001296726.1 NZ_CP053723:2484472-2484738 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT157565 NZ_CP069372:36581-36808 [Neotabrizicola shimadae]
ATGCAGGTCGCGAAATGGGGCAACTCGCTTGCCGTCCGTTTGCCCGCCGAGCTTGTCCGGGAACTTGGCCTCAAGGAAGG
TGATCAGATCGACCTGGTCAAGGACGACGGCCAGGTAAGAGTCCGTCGTCTGGCGCGTGCGGATGAGGTGCTGACGGGTC
TGCGCCGCTTTAGGGGCAAGCTTCCCGCTGCGGAACGCCTGAGCCGCGAAGACGCGCATGAGCGCTGA

Structures

Toxin

• AlphaFold DB: E0IX85