Detailed information of TA system TA157556

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1958323..1959113
• Replicon: chromosome
• Accession: NZ_CP053723
• Organism: Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1L4HFA4
• Locus tag: HP433_RS09425
• Protein ID: WP_032163901.1
• Coordinates: 1958323..1958697 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HP433_RS09430
• Protein ID: WP_170936813.1
• Coordinates: 1958787..1959113 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP433_RS09385	1953734..1954900	+	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HP433_RS09390	1955019..1955492	+	474	WP_001105389.1	DNA gyrase inhibitor SbmC	-
HP433_RS09395	1955690..1956748	+	1059	WP_001200910.1	FUSC family protein	-
HP433_RS09400	1956920..1957249	+	330	WP_000450409.1	DUF496 family protein	-
HP433_RS09405	1957350..1957532	-	183	WP_001016348.1	hypothetical protein	-
HP433_RS09410	1957604..1957732	+	129	Protein_1842	transposase domain-containing protein	-
HP433_RS09415	1958021..1958134	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HP433_RS09420	1958147..1958326	-	180	WP_053902470.1	hypothetical protein	-
HP433_RS09425	1958323..1958697	-	375	WP_032163901.1	TA system toxin CbtA family protein	Toxin
HP433_RS09430	1958787..1959113	-	327	WP_170936813.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HP433_RS09435	1959229..1959450	-	222	WP_000692318.1	DUF987 domain-containing protein	-
HP433_RS09440	1959519..1959995	-	477	WP_172615454.1	RadC family protein	-
HP433_RS09445	1960011..1960496	-	486	WP_000849582.1	antirestriction protein	-
HP433_RS09450	1960551..1961369	-	819	WP_040061766.1	DUF945 domain-containing protein	-
HP433_RS09455	1961469..1961702	-	234	WP_001119729.1	DUF905 family protein	-
HP433_RS09460	1961781..1962236	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13919.89 Da
• Isoelectric Point: 7.7760

>T157556 WP_032163901.1 NZ_CP053723:c1958697-1958323 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T157556 NZ_CP069355:c278455-278204 [Microvirga sp. VF16]
ATGAAGAAGATTGCTTACAGCAAATCGGCCCTGAAGACCCTGCGTCGGATTCCAGCAAATGTCTCAGCCACGATCAGAGG
TAAGATCGAGCAATATGCCTCCGATCCAGCCTCGCTCTCGGCCAACGTGACGAAGCTACAAGGGCGCGAGGGCTATCGGT
TACGGGTCGGGGATTGGCGCGTGATCTTTGATGAGGACGGCACTGTTCTGGCCATCCTGGAAATCGGCCCCAGGGGCGGA
ATTTACGGCTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12007.84 Da
• Isoelectric Point: 7.7960

>AT157556 WP_170936813.1 NZ_CP053723:c1959113-1958787 [Escherichia coli]
ITPPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDTDAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYA
KGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT157556 NZ_CP069355:c278194-277820 [Microvirga sp. VF16]
ATGAATAAGCCTGACATTATTACGACCCCGAGTGGCGACCGTTTGGCGATCATGCCGCTTGCGGATTATGAAAGCCTGGT
CGCCTCGGCGGAGCAGTCTGCCGACGTGCGCGCCTACGATGAGGCAAAGCGGCGGATCGCCAGCGGCGAAGACGAGCTTG
TTCCGAGCGAGTTCGCCAACCGCATTCTCGACGGCGAGAGCCCGGTTCGGGTTTGGCGCGAGTATCGCGGCATGAGCGCA
AAAGACCTTGCCGCCAAGACCGGTCTCAGCCCGGCTTATATTAGTCAAATTGAGACTGGTGATCGCGACGGCAGTTTCGA
GACCATGAAGAAGATCGCGCAAGCCTTGTCGGTGTCGCTCGACGACCTTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A1L4HFA4