Detailed information of TA system TA157554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1362691..1363316
• Replicon: chromosome
• Accession: NZ_CP053723
• Organism: Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HP433_RS06520
• Protein ID: WP_000911330.1
• Coordinates: 1362918..1363316 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HP433_RS06515
• Protein ID: WP_000450524.1
• Coordinates: 1362691..1362918 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP433_RS06490	1358494..1358964	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HP433_RS06495	1358964..1359536	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HP433_RS06500	1359682..1360560	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HP433_RS06505	1360577..1361611	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
HP433_RS06510	1361824..1362537	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HP433_RS06515	1362691..1362918	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HP433_RS06520	1362918..1363316	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HP433_RS06525	1363463..1364326	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
HP433_RS06530	1364341..1366356	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
HP433_RS06535	1366430..1367128	+	699	WP_000679812.1	esterase	-
HP433_RS06540	1367209..1367409	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T157554 WP_000911330.1 NZ_CP053723:1362918-1363316 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T157554 NZ_CP069354:c4012295-4011882 [Microvirga sp. VF16]
ATGCCCGAACCCCGATATCTCCTCGATACCAATATCTGCATCTACATTCGCCGGGAACGCCCACCATCCGTGCTGGATCG
GTTCAAGGCCTTGAACCCCGGCTCGACGGTGATCTCCGTCATCACCTATGGTGAGCTGGTCTACGGGGCCCGCAAAAGCC
AGGACTCCGACAAAGCGATGATGATCTTGGACGAGCTCACAGAGCTCATTCCCGTGGTCCCCATGGCGACGGATGTGGCA
GACACCTATGGACTGATTCGGTCTGATCTGTCGGCGCGCGGTGCCTTGATTGGCAACAATGATCTTTGGATCGCAGCCCA
TGCCATGAGCCTGAACTTAACTCTCGTGACAAACAATGAGAAAGAGTTTCAGCGGGTTGGCGGCCTCACACTCGAAAACT
GGGCCAAGAGATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT157554 WP_000450524.1 NZ_CP053723:1362691-1362918 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT157554 NZ_CP069354:c4012513-4012295 [Microvirga sp. VF16]
ATGGCATTCGCTAAGGTTTTCCGGTCTGGCAACAGCCAAGCCGTTCGGCTGCCCAAACAATTTCGCCTCACGAGCGACGA
GGTCGAGATTTTCCGCCGCGGCGACGAGATCGTCCTGCGCGAGAAGCCCAGAAGCCTGGCACGCGCCTTCGAATTGCTGT
GTGATCTTCCGGAAGTCGAGCGCGACGATGCTCCCCCTCAGGAGCGTAAGGGCCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33