Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 933351..934005 Replicon chromosome
Accession NZ_CP053723
Organism Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HP433_RS04475 Protein ID WP_000244772.1
Coordinates 933598..934005 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HP433_RS04470 Protein ID WP_000354046.1
Coordinates 933351..933617 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP433_RS04445 928639..929382 + 744 WP_000951964.1 SDR family oxidoreductase -
HP433_RS04450 929439..930872 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
HP433_RS04455 930917..931228 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HP433_RS04460 931392..932051 + 660 WP_000250274.1 hemolysin III family protein -
HP433_RS04465 932128..933108 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HP433_RS04470 933351..933617 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HP433_RS04475 933598..934005 + 408 WP_000244772.1 protein YgfX Toxin
HP433_RS04480 934045..934566 - 522 WP_001055874.1 flavodoxin FldB -
HP433_RS04485 934678..935574 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HP433_RS04490 935599..936309 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HP433_RS04495 936315..938048 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T157552 WP_000244772.1 NZ_CP053723:933598-934005 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T157552 NZ_CP069353:1047132-1047413 [Saccharopolyspora erythraea]
ATGCTGCGATCTTTCGCTGACAAGGAGACCGAGCGTGTATGGCAACGACGACGTTCGCGGATGCTCGATCCGAAGGTTCA
GCGGGCGGCTCTGCGTAAGCTCGCCATCCTTGATGCGGCGGAGACCGTGGAAGACCTGCGAGTCCCACCTGGAAACCAAC
TGGAGAAGCTGAACGGAGATCGGGTCGGCCAGTACAGCATCCGGATCAACCAGCAGTGGCGGATCCGCTTTGAGTGGACA
TCAGCCGGGCCCGAGAACGTCGAGATCGTGGACTACCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT157552 WP_000354046.1 NZ_CP053723:933351-933617 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT157552 NZ_CP069353:1047426-1047725 [Saccharopolyspora erythraea]
ATGTCTGACGAGTGCGTCATGCCTCCGGTTCATCCCGGGGAAGTCCTGGCCGAGGAGTACTTGGAGCCGCTCGGCGTCAC
ACAGCACCGGTTGGCCGTGGCGATCGGCGTTCCGCCGCGCCGGATCAACGAGATCGTGCACGGCAAGCGTGGGATCTCGG
CCGACACGGCGCTGCGGCTCGCGCGCTTCTTCGGCACCTCCGAGAGGTTCTGGCTCAATCTTCAGTCGCGCTACGACTTG
GAACGTGAGAGGGACGCCCTCGCTGACACGTTGGACCGGATCCAACCGCTGTCCGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References