Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 298559..299190 Replicon chromosome
Accession NZ_CP053723
Organism Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag HP433_RS01310 Protein ID WP_001259388.1
Coordinates 298559..298834 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HP433_RS01315 Protein ID WP_000593555.1
Coordinates 298831..299190 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP433_RS01295 293563..294630 + 1068 WP_000361485.1 HlyD family secretion protein -
HP433_RS01300 294627..297362 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
HP433_RS01305 297362..298486 + 1125 WP_001314210.1 ABC transporter permease -
HP433_RS01310 298559..298834 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
HP433_RS01315 298831..299190 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HP433_RS01320 299310..299711 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HP433_RS01325 299717..300523 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
HP433_RS01330 300520..301284 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HP433_RS01335 301284..302117 - 834 WP_106464827.1 nickel ABC transporter permease subunit NikC -
HP433_RS01340 302114..303058 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 283530..299190 15660


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T157549 WP_001259388.1 NZ_CP053723:298559-298834 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T157549 NZ_CP069352:5281452-5281706 [Pseudomonas granadensis]
ATGCAAACCATCAACTACACAACCGCCCGTGCGCACTTGGCCGAGACGATGGACCGTGTCAATGAAGACCGTGCGCCTCT
ACTGGTTACCCGGCAGAAAGGGGAACCTGTTGTGATGATGTCCCTGTCCGATTACAACGCGCTAGAAGAAACAGCCTATT
TGCTGCGCTCACCTGCCAACGCCGAGCGCTTGATGAAGTCCATCAACGAGCTGCGCGCCGGTAAAGCCAAAGCCAGGCAA
CTGATTGAAGAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT157549 WP_000593555.1 NZ_CP053723:298831-299190 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT157549 NZ_CP069352:5281703-5281957 [Pseudomonas granadensis]
ATGAAAATCCAGTTCACGCCCACGGGCTGGGAAGACTATTTGTGGTTTCAACAGAACGACAAGGCCGGACTCAAGCGCAT
CAATCTCCTGATCAAAGCGATCAAGCGCGAACCATTTGAGGGCGTCGGAAAGCCAGAGGCGCTCAAGCACAACATGAGCG
GCCTCTGGTCTCGCCGGATAACTGCGGAGCATCGTCTGGTTTACATGATTGAGGACGGCGAGGTGTGCGTCATCAGCTGC
AGATACCACTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References