Detailed information of TA system TA157544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 45615..46150
• Replicon: plasmid pCP131-IncFIB
• Accession: NZ_CP053722
• Organism: Escherichia coli strain CP131_Sichuan

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HP434_RS23345
• Protein ID: WP_001270421.1
• Coordinates: 45615..45902 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: HP434_RS23350
• Protein ID: WP_001132895.1
• Coordinates: 45899..46150 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP434_RS23320	41125..41822	+	698	Protein_47	IS1 family transposase	-
HP434_RS23325	42274..42441	+	168	WP_162841962.1	transcriptional regulator	-
HP434_RS23330	42562..43302	+	741	WP_001066941.1	tyrosine-type recombinase/integrase	-
HP434_RS23335	43581..44558	-	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
HP434_RS23340	45202..45613	-	412	Protein_51	DDE-type integrase/transposase/recombinase	-
HP434_RS23345	45615..45902	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HP434_RS23350	45899..46150	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HP434_RS23355	46324..46536	-	213	WP_000402944.1	hypothetical protein	-
HP434_RS23360	46911..48116	+	1206	WP_000817642.1	AAA family ATPase	-
HP434_RS23365	48113..49075	+	963	WP_032200967.1	ParB/RepB/Spo0J family partition protein	-
HP434_RS23370	49216..49389	-	174	Protein_57	DUF4113 domain-containing protein	-
HP434_RS23375	49586..49888	+	303	WP_122985717.1	antirestriction protein	-
HP434_RS23380	49925..51093	-	1169	Protein_59	IS200/IS605 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul3 / mph(A) / floR / blaTEM-1B / tet(M) / aadA2 / dfrA12	-	1..121655	121655
	-	flank	IS/Tn	-	-	49925..50917	992

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T157544 WP_001270421.1 NZ_CP053722:c45902-45615 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T157544 NZ_CP069352:2477289-2477564 [Pseudomonas granadensis]
ATGGAGTTGAAGTGGACGAGCAAAGGACTTTCCGATTTGACCAGGCTTTACGAATTCTTGGCAGCAGTCAATCAACCTGC
CGCTGCACGTACTGTGCAACAGCTGACTGCTGCTCCTGCAATGCTATTGGCCAATCCGCGCTTGGGCGAGCGTCTGGAAG
AGTTTGAACCGCGAGACGTGCGAAGGATTCTGGTAGGGCACTACGACATGCGCTACGAGATTATCGGTCCGACTATCTAC
CTGCTGCGCCTTTGGCACACCCGCGAAGAACGTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT157544 WP_001132895.1 NZ_CP053722:c46150-45899 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT157544 NZ_CP069352:2477015-2477287 [Pseudomonas granadensis]
ATGGCCGCGACAATGAAAACCCGATCCGTTACTGCCCACGTTCCGGTGCAGTTGGCCGAGAAGGTCGACCTGATGGCCGA
GCGTCTGGAACGCTCGCGCAACTGGATCGTGAAACAAGCCCTGAGCGCCTGGATTGATCAAGAAGAAGAACGCAGCCGTT
TGACGCGCGAAGCGTTGGCTGATGTGGACGCCGGGCGCGTCATCGATCATCAATCGGTGCAGGCTTGGGCGGACAGCTTG
AGTTCTGATGCTCCGCTCAAGGCACCACTTTAA

Structures

Antitoxin

• AlphaFold DB: L4J0V6