Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4086466..4086963 Replicon chromosome
Accession NZ_CP053720
Organism Escherichia coli strain CP131_Sichuan

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HP434_RS19590 Protein ID WP_001173343.1
Coordinates 4086466..4086639 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HP434_RS19595 Protein ID WP_000398619.1
Coordinates 4086667..4086963 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP434_RS19580 4083131..4084588 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HP434_RS19585 4084825..4086342 + 1518 WP_001295074.1 lysine--tRNA ligase -
HP434_RS19590 4086466..4086639 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HP434_RS19595 4086667..4086963 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HP434_RS19600 4087190..4087462 - 273 WP_000405647.1 N-acetyltransferase -
HP434_RS19605 4087474..4087704 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
HP434_RS19610 4087885..4089516 + 1632 WP_086625171.1 sensor histidine kinase -
HP434_RS19615 4089513..4090232 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T157543 WP_001173343.1 NZ_CP053720:c4086639-4086466 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T157543 NZ_CP069352:c1368707-1368396 [Pseudomonas granadensis]
ATGGAAAAGAACACACCCCATTACGACTTGGCGGTGATCAAGGCGGATGTCAGGAGGCTAGGCTGGAGAGCGTTCACGCA
CACTGCCAGAAGAAGCGGATCTGAGCTGGAGCTGACGCTCGCAGACATGCAGGAAGTAGTTTTCAGGCTGCAGCGCAGCA
TGCTCTACAAGTCGATGACGACTTATGACAACCACCGTATTTGGCAGGATGTCTATCACATCAATTCACATGGTCTGGAG
ATTTACATAAAGGTGTCATATCACTACGACAAGCGCCCACCTGTGATCTCTTTCAAGGAGAACAACTCATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT157543 WP_000398619.1 NZ_CP053720:c4086963-4086667 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT157543 NZ_CP069352:c1368399-1367917 [Pseudomonas granadensis]
ATGAGTACGCAACAGTGTTTCAGTTGCGGTGCCGCTGAAGGCATGGTGCATTTCGACGGCCGAGGCGAAACGGTGAGCGT
CAAAGGGATGGAGCGCCGGATTGACAACCTGTCGGGGTGGGAATGTCAGAAGTGCGGAGAGGTCGAGCTCGATGAAATGT
GCGGGCAACGTTATTCAGATGCATGCGATGAACTCGTTATCGCCAGCAGGAAAATGGTTGGCAACGAGATGAGAAGAATT
CGCCGCAAATTGCATCTGACGCAAAAAGAGACGGTGCAACTGCTGTCTGGGGGTGGGCATAACGCATTCTCTCGCTACGA
GCGCGGCGAAATCTTGCCGCCCAAGGCACTCATACTGTTGATGCGTTTATTGGATCGTTACCCGTATTTGCTAACCGATG
CGAAGACTCTCGGCGAAGGTGCAGATTTGAGAGGTTTCGAGCACATCGTTCACAAAGAGCATGAATCCCTTTCGGTTTCC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References