Detailed information of TA system TA157516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 909568..910589
• Replicon: chromosome
• Accession: NZ_CP053718
• Organism: Proteus mirabilis strain MPE4069

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HN016_RS04060
• Protein ID: WP_004249488.1
• Coordinates: 909568..910137 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: HN016_RS04065
• Protein ID: WP_004245528.1
• Coordinates: 910137..910589 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HN016_RS04055	907098..909452	-	2355	WP_017628571.1	DNA polymerase II	-
HN016_RS04060	909568..910137	-	570	WP_004249488.1	PIN domain-containing protein	Toxin
HN016_RS04065	910137..910589	-	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
HN016_RS04070	910717..911418	-	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
HN016_RS04075	911411..912991	-	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
HN016_RS04080	912991..914004	-	1014	WP_004250228.1	thiamine ABC transporter substrate binding subunit	-
HN016_RS04085	914273..914803	+	531	WP_004245524.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T157516 WP_004249488.1 NZ_CP053718:c910137-909568 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T157516 NZ_CP069333:3408131-3408709 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT157516 WP_004245528.1 NZ_CP053718:c910589-910137 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT157516 NZ_CP069333:3407488-3408042 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3