Detailed information of TA system TA157509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1292884..1293801
• Replicon: chromosome
• Accession: NZ_CP053717
• Organism: Bacillus velezensis strain A2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: HNV93_RS05745
• Protein ID: WP_007407256.1
• Coordinates: 1292884..1293630 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HNV93_RS05750
• Protein ID: WP_003154807.1
• Coordinates: 1293631..1293801 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNV93_RS05725	1288277..1289227	-	951	WP_032874618.1	ring-cleaving dioxygenase	-
HNV93_RS05730	1289549..1290865	+	1317	WP_032874616.1	amino acid permease	-
HNV93_RS05735	1291151..1291768	+	618	WP_032874614.1	DUF47 domain-containing protein	-
HNV93_RS05740	1291781..1292779	+	999	WP_032874611.1	inorganic phosphate transporter	-
HNV93_RS05745	1292884..1293630	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HNV93_RS05750	1293631..1293801	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HNV93_RS05755	1294058..1294936	-	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
HNV93_RS05760	1294950..1295213	-	264	WP_003154813.1	phage holin	-
HNV93_RS05765	1295227..1295490	-	264	WP_003154815.1	hemolysin XhlA family protein	-
HNV93_RS05770	1295542..1296303	-	762	WP_032874607.1	hypothetical protein	-
HNV93_RS05775	1296360..1296557	-	198	WP_032874605.1	XkdX family protein	-
HNV93_RS05780	1296562..1296933	-	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
HNV93_RS05785	1296946..1298568	-	1623	WP_032874601.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T157509 WP_007407256.1 NZ_CP053717:1292884-1293630 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T157509 NZ_CP069332:1501329-1501607 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT157509 WP_003154807.1 NZ_CP053717:1293631-1293801 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT157509 NZ_CP069332:1501619-1501924 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14