Detailed information of TA system TA157502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3293236..3293783
• Replicon: chromosome
• Accession: NZ_CP053708
• Organism: Lichenicola cladoniae strain PAMC 26569

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HN018_RS14960
• Protein ID: WP_171833303.1
• Coordinates: 3293460..3293783 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HN018_RS14955
• Protein ID: WP_171833304.1
• Coordinates: 3293236..3293463 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HN018_RS14940 (HN018_14950)	3289713..3290957	-	1245	WP_171833307.1	AGE family epimerase/isomerase	-
HN018_RS14945 (HN018_14955)	3290957..3292441	-	1485	WP_171833306.1	D-glycero-beta-D-manno-heptose-7-phosphate kinase	-
HN018_RS14950 (HN018_14960)	3292452..3293105	-	654	WP_171833305.1	guanylate kinase	-
HN018_RS14955 (HN018_14965)	3293236..3293463	+	228	WP_171833304.1	antitoxin MazE family protein	Antitoxin
HN018_RS14960 (HN018_14970)	3293460..3293783	+	324	WP_171833303.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HN018_RS14965 (HN018_14975)	3293790..3295364	-	1575	WP_171833302.1	2-isopropylmalate synthase	-
HN018_RS14970 (HN018_14980)	3295813..3296364	+	552	WP_171833301.1	glycine zipper family protein	-
HN018_RS14975 (HN018_14985)	3296377..3297186	-	810	WP_239479317.1	N-acetylmuramoyl-L-alanine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• Antitoxin MazE-like (8-73)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11678.73 Da
• Isoelectric Point: 8.9365

>T157502 WP_171833303.1 NZ_CP053708:3293460-3293783 [Lichenicola cladoniae]
VRRGDLVTVALQGDQGKPRPALIIQGDLFHDLHSVTILPITSMLLDAPLTRIMVQPNDRNGLTKPSQIMVDKPQTPARIR
LGPVFGELDDLTMIAVNRAVALFFGLA

Nucleotide

• Length: 324 bp

>T157502 NZ_CP069331:c143237-142956 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8400.44 Da
• Isoelectric Point: 9.2694

>AT157502 WP_171833304.1 NZ_CP053708:3293236-3293463 [Lichenicola cladoniae]
MTATGTGAERVRRRRDKLRAAGLRPVQIWVPDTRAPGFALECARQSRIIRDAELQAGHTEYESWSAVSDMDGWTA

Nucleotide

• Length: 228 bp

>AT157502 NZ_CP069331:c143461-143234 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA