Detailed information of TA system TA157492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1946957..1947999
• Replicon: chromosome
• Accession: NZ_CP053706
• Organism: Pseudomonas aeruginosa strain PAC1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HPT09_RS08745
• Protein ID: WP_003153636.1
• Coordinates: 1946957..1947532 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: HPT09_RS08750
• Protein ID: WP_003050245.1
• Coordinates: 1947529..1947999 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPT09_RS08720	1943395..1944120	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
HPT09_RS08725	1944159..1945061	-	903	WP_003153640.1	nucleotidyltransferase domain-containing protein	-
HPT09_RS08730	1945061..1945561	-	501	WP_003090159.1	hypothetical protein	-
HPT09_RS08735	1945558..1946028	-	471	WP_003153638.1	hypothetical protein	-
HPT09_RS08740	1946025..1946939	-	915	WP_003050256.1	AAA family ATPase	-
HPT09_RS08745	1946957..1947532	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
HPT09_RS08750	1947529..1947999	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
HPT09_RS08755	1948203..1948586	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
HPT09_RS08760	1948583..1948816	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
HPT09_RS08765	1948833..1949192	+	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
HPT09_RS08770	1949205..1949603	+	399	WP_003153632.1	TIGR03750 family conjugal transfer protein	-
HPT09_RS08775	1949600..1950292	+	693	WP_004350603.1	TIGR03746 family integrating conjugative element protein	-
HPT09_RS08780	1950289..1951200	+	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
HPT09_RS08785	1951190..1952608	+	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T157492 WP_003153636.1 NZ_CP053706:c1947532-1946957 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T157492 NZ_CP069329:2362062-2362817 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT157492 WP_003050245.1 NZ_CP053706:c1947999-1947529 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT157492 NZ_CP069329:2361559-2361927 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCTTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Antitoxin

• AlphaFold DB: I3TV68