Detailed information of TA system TA157491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 752986..754207
• Replicon: chromosome
• Accession: NZ_CP053706
• Organism: Pseudomonas aeruginosa strain PAC1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: HPT09_RS03545
• Protein ID: WP_003102740.1
• Coordinates: 752986..753564 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HPT09_RS03550
• Protein ID: WP_003088112.1
• Coordinates: 753653..754207 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPT09_RS03530	748262..749539	+	1278	WP_003102743.1	TolC family protein	-
HPT09_RS03535	749529..751700	+	2172	WP_003114950.1	type I secretion system permease/ATPase	-
HPT09_RS03540	751690..752877	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HPT09_RS03545	752986..753564	-	579	WP_003102740.1	HD domain-containing protein	Toxin
HPT09_RS03550	753653..754207	-	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HPT09_RS03555	754365..756560	-	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HPT09_RS03560	756565..757026	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HPT09_RS03565	757357..757680	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
HPT09_RS03570	757693..758091	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HPT09_RS03575	758088..758639	-	552	WP_004343115.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T157491 WP_003102740.1 NZ_CP053706:c753564-752986 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T157491 NZ_CP069329:1501244-1501522 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT157491 WP_003088112.1 NZ_CP053706:c754207-753653 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT157491 NZ_CP069329:1501534-1501839 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A0C7CS07