Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 359630..360201 Replicon chromosome
Accession NZ_CP053704
Organism Enterococcus faecium strain FS 86

Toxin (Protein)


Gene name mazF Uniprot ID R2Q2D5
Locus tag HPK29_RS01715 Protein ID WP_002341043.1
Coordinates 359860..360201 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2A4E1M8
Locus tag HPK29_RS01710 Protein ID WP_025477367.1
Coordinates 359630..359860 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK29_RS01690 355894..356487 + 594 WP_002342793.1 malonate decarboxylase holo-ACP synthase -
HPK29_RS01695 356511..357596 + 1086 WP_002342794.1 acyltransferase family protein -
HPK29_RS01700 357618..358487 + 870 WP_002342795.1 triphosphoribosyl-dephospho-CoA synthase MdcB -
HPK29_RS01705 358599..359363 + 765 WP_002341045.1 TIM44-like domain-containing protein -
HPK29_RS01710 359630..359860 + 231 WP_025477367.1 hypothetical protein Antitoxin
HPK29_RS01715 359860..360201 + 342 WP_002341043.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HPK29_RS01720 360381..360740 + 360 WP_156238084.1 hypothetical protein -
HPK29_RS01725 360917..362998 + 2082 WP_002342796.1 BglG family transcription antiterminator -
HPK29_RS01730 363034..363336 + 303 Protein_345 PTS sugar transporter subunit IIB -
HPK29_RS01735 363362..364651 + 1290 WP_002341041.1 PTS sugar transporter subunit IIC -
HPK29_RS01740 364670..365077 + 408 Protein_347 tetratricopeptide repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13316.66 Da        Isoelectric Point: 9.9258

>T157483 WP_002341043.1 NZ_CP053704:359860-360201 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQ
LKSLDFKERRLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T157483 NZ_CP069327:c5247083-5246805 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8566.42 Da        Isoelectric Point: 4.0070

>AT157483 WP_025477367.1 NZ_CP053704:359630-359860 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL

Download         Length: 231 bp

>AT157483 NZ_CP069327:c5246793-5246488 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829AD04

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4E1M8

References