Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32135..32772 Replicon plasmid pEh27_5
Accession NZ_CP053696
Organism Enterobacter hormaechei strain Eh27

Toxin (Protein)


Gene name vagD Uniprot ID A0A9E6WXZ4
Locus tag HPC66_RS24565 Protein ID WP_032662169.1
Coordinates 32135..32545 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A9E6WTF5
Locus tag HPC66_RS24570 Protein ID WP_032662167.1
Coordinates 32542..32772 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPC66_RS24545 (HPC66_24490) 27501..27851 + 351 WP_032662182.1 DUF305 domain-containing protein -
HPC66_RS24550 (HPC66_24495) 28037..28945 - 909 WP_032662179.1 HNH endonuclease -
HPC66_RS24555 (HPC66_24505) 29492..30514 - 1023 WP_032662174.1 helicase UvrD -
HPC66_RS24560 (HPC66_24510) 30499..32061 - 1563 WP_032662171.1 AAA family ATPase -
HPC66_RS24565 (HPC66_24515) 32135..32545 - 411 WP_032662169.1 PIN domain-containing protein Toxin
HPC66_RS24570 (HPC66_24520) 32542..32772 - 231 WP_032662167.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HPC66_RS24575 (HPC66_24525) 33314..33604 + 291 WP_013087126.1 hypothetical protein -
HPC66_RS24580 (HPC66_24530) 33735..34265 + 531 WP_013087127.1 hypothetical protein -
HPC66_RS24585 (HPC66_24535) 34284..35060 + 777 WP_015572054.1 site-specific integrase -
HPC66_RS24590 (HPC66_24540) 35262..35519 + 258 Protein_36 site-specific integrase -
HPC66_RS24595 (HPC66_24545) 35763..36773 - 1011 WP_015572055.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B - 1..79223 79223


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-127)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14729.03 Da        Isoelectric Point: 5.7351

>T157478 WP_032662169.1 NZ_CP053696:c32545-32135 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Download         Length: 411 bp

>T157478 NZ_CP069327:c143234-142953 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8645.77 Da        Isoelectric Point: 4.4692

>AT157478 WP_032662167.1 NZ_CP053696:c32772-32542 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Download         Length: 231 bp

>AT157478 NZ_CP069327:c143458-143231 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References