Detailed information of TA system TA157460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 891612..892261
• Replicon: chromosome
• Accession: NZ_CP053685
• Organism: Proteus mirabilis strain MPE5203

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: HOY68_RS03920
• Protein ID: WP_012368534.1
• Coordinates: 891612..892031 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: HOY68_RS03925
• Protein ID: WP_012368535.1
• Coordinates: 892028..892261 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HOY68_RS03890	886890..887768	-	879	WP_004246812.1	23S rRNA pseudouridine(2604) synthase RluF	-
HOY68_RS03895	887944..888858	-	915	WP_004246811.1	fatty acid biosynthesis protein FabY	-
HOY68_RS03900	888882..889319	-	438	WP_004246810.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HOY68_RS03905	889400..890017	-	618	WP_004246809.1	glucose-1-phosphatase	-
HOY68_RS03910	890670..891086	-	417	WP_046334846.1	hypothetical protein	-
HOY68_RS03915	891064..891387	-	324	WP_046334845.1	hypothetical protein	-
HOY68_RS03920	891612..892031	-	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
HOY68_RS03925	892028..892261	-	234	WP_012368535.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HOY68_RS03930	893552..893752	+	201	WP_104836792.1	hypothetical protein	-
HOY68_RS03935	894010..894960	-	951	WP_104836793.1	reverse transcriptase family protein	-
HOY68_RS03940	894953..895099	-	147	WP_158660334.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T157460 WP_012368534.1 NZ_CP053685:c892031-891612 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T157460 NZ_CP069324:c143237-142956 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT157460 WP_012368535.1 NZ_CP053685:c892261-892028 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT157460 NZ_CP069324:c143461-143234 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0