Detailed information of TA system TA157451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3371041..3371840
• Replicon: chromosome
• Accession: NZ_CP053683
• Organism: Proteus mirabilis strain MPE0027

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: HNV02_RS15885
• Protein ID: WP_004247966.1
• Coordinates: 3371316..3371840 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: HNV02_RS15880
• Protein ID: WP_004247964.1
• Coordinates: 3371041..3371319 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNV02_RS15855	3366391..3367473	+	1083	WP_004242680.1	peptide chain release factor 1	-
HNV02_RS15860	3367473..3368321	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
HNV02_RS15865	3368299..3369114	+	816	WP_004242688.1	invasion regulator SirB1	-
HNV02_RS15870	3369172..3370026	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
HNV02_RS15875	3370034..3370963	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
HNV02_RS15880	3371041..3371319	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
HNV02_RS15885	3371316..3371840	+	525	WP_004247966.1	hypothetical protein	Toxin
HNV02_RS15890	3371921..3373621	-	1701	WP_017628159.1	C4-dicarboxylic acid transporter DauA	-
HNV02_RS15900	3374585..3375526	+	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T157451 WP_004247966.1 NZ_CP053683:3371316-3371840 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T157451 NZ_CP069319:c4818323-4817946 [Kosakonia cowanii]
ATGGTGACTATGAGCAACCTTGCGTTATTTGATACCAATATCTTGATTGATCTGTTTAATGGCCGGGAAGAGGCTGCTAA
CACTTTTGAAGATTACAATGGCAGGATCGCCATCAGCCTGATTAGCTGGATTGAAGTGCTGGTTGGCGCTAAAAAAGTAG
GGCAGGAAAATGCGACACGCGCTTTTCTTGATTCGCTTGAGGTCATAGAGATTTCCCATCCCATAGCAGAACGAAGCATT
GAGCTGCGCCAGGCGTATCGCATGAAGCTGCCCGATGCCATTATTCTTGCAACAGCTCAGATACATAGCCGTTGCCTCGT
TACGCGCAATATCAAAGATTTTGCGTCTGTAACAGGGGTTTTCACCCCATATCAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT157451 WP_004247964.1 NZ_CP053683:3371041-3371319 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT157451 NZ_CP069319:c4818559-4818317 [Kosakonia cowanii]
ATGGATGTTCTCTCTGGAGTGATTATGGGGCGTATTTTGTTGGATTTATCAGATGATGTTATACGCAGGCTCGATAGCTT
AAAGCAGCAGCGTAACCTTCCTCGTGCTGAATTATTACGAGAAGCGGTTGAGCAGTATCTTGAAAAGCAGAACACGACAA
GCGCTCGCAATGCTTTAGGTCTATGGGGAGGTGAGGGCGAAGATGGTCTTGAATTTGAGAGAAAACTGCGCGAGGAATGG
TGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2