Detailed information of TA system TA157446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 1236530..1237229
• Replicon: chromosome
• Accession: NZ_CP053683
• Organism: Proteus mirabilis strain MPE0027

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HNV02_RS05680
• Protein ID: WP_017628658.1
• Coordinates: 1236530..1236916 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: HNV02_RS05685
• Protein ID: WP_004246828.1
• Coordinates: 1236909..1237229 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNV02_RS05665	1232549..1233130	-	582	WP_012368524.1	DNA-3-methyladenine glycosylase I	-
HNV02_RS05670	1233381..1234286	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
HNV02_RS05675	1234296..1236368	+	2073	WP_017628659.1	glycine--tRNA ligase subunit beta	-
HNV02_RS05680	1236530..1236916	+	387	WP_017628658.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HNV02_RS05685	1236909..1237229	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
HNV02_RS05690	1237278..1237712	-	435	WP_004246827.1	hypothetical protein	-
HNV02_RS05695	1237705..1238589	-	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
HNV02_RS05700	1238804..1240090	-	1287	WP_017628657.1	DUF3748 domain-containing protein	-
HNV02_RS05705	1240227..1240844	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
HNV02_RS05710	1241145..1241768	+	624	WP_004246821.1	guanylate kinase	-
HNV02_RS05715	1241823..1242098	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14322.65 Da
• Isoelectric Point: 10.0826

>T157446 WP_017628658.1 NZ_CP053683:1236530-1236916 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVRYD

Nucleotide

• Length: 387 bp

>T157446 NZ_CP069319:3652777-3652995 [Kosakonia cowanii]
ATGTCCGAAAAACCATTAACAAAAATTGATTATTTGATGCGTTTAAGGCGCTGCCAATCAATTGATACGCTTGAACGCGT
TATTGAAAAAAATAAATACGAACTGACCGATGATGAGCTTACGGTGTTTTATTCAGCGGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTTTATGACAAAATCCCCTCGTCGGTGTGGAAATTTGTTCGCTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT157446 WP_004246828.1 NZ_CP053683:1236909-1237229 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT157446 NZ_CP069319:3652379-3652753 [Kosakonia cowanii]
ATGGACGAATACTCGCCGAAAAGGCATGATATTGCGCAGCTTAAATTTCTCTGCGAATCGTTGCACCAGGAGTGCCTCGC
CAATCTGGAAGAGAGCCATCATGGATGGGTTAACGATCCCACATCAGCCATCAACCTGCAGTTAAATGAGCTGATTGAGC
ATATTGCAACCTTCGCGCTGAATTATAAAATTAAGCACGACAAAGATAATAAGCTGATTGAATTGATCGACGAATACCTT
GATGACACGTTTATGTTGTTTAGCAGTTACGGTATTAATACACACGACTTACAGAAATGGCGTAAAACAGGCAGCAAGCT
GTTTCGCAGTTTCGTCAATGTAAGCAAGGCTAATCCAGTAAGTCACTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: B4EZ97