Detailed information of TA system TA157444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 705757..706778
• Replicon: chromosome
• Accession: NZ_CP053683
• Organism: Proteus mirabilis strain MPE0027

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HNV02_RS03345
• Protein ID: WP_004249488.1
• Coordinates: 706209..706778 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: HNV02_RS03340
• Protein ID: WP_004245528.1
• Coordinates: 705757..706209 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNV02_RS03320	701543..702073	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
HNV02_RS03325	702342..703355	+	1014	WP_004250228.1	thiamine ABC transporter substrate binding subunit	-
HNV02_RS03330	703355..704935	+	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
HNV02_RS03335	704928..705629	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
HNV02_RS03340	705757..706209	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
HNV02_RS03345	706209..706778	+	570	WP_004249488.1	PIN domain-containing protein	Toxin
HNV02_RS03350	706894..709248	+	2355	WP_017628571.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T157444 WP_004249488.1 NZ_CP053683:706209-706778 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T157444 NZ_CP069319:821835-822251 [Kosakonia cowanii]
GTGGTCCTGTGGCAATCTGAGTTACGCGTCTCCTGGCGCGCACAGTGGCTCTCCTTATTGCTCCATGGATTCGTAGCCGC
ACTCATCTTGCTGATGCCCTGGCCGCTGAGCTACACGCCGGTATGGCTGCTGCTGCTTTCGCTGGTGGTGTTTGACTCCG
TACGGAGCCAACGGCGCATCAACGCCTGTCATGGCGAGATTAAGCTGCTGATGGATTCCCGCCTGCGCTGGCAGGGCGAA
GAGTGGGATATTGTCGGTACGCCCTGGATGCTGCGTAGCGGGATGATGCTGCGTTTGCGTCGCGAAGAAGATGGGCGTCG
GCAGCATCTCTGGCTGGCATCAGACAGTATGGATGCGCAGGAGTGGCGCGATCTGCGCCGGATGATTTTACAAAAACCGG
CGCAGGGTCTGCACTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT157444 WP_004245528.1 NZ_CP053683:705757-706209 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT157444 NZ_CP069319:821588-821854 [Kosakonia cowanii]
ATGGATATTACTAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGAATGCGCGAGCTCGATATTTCCATCATGCCTTT
TTTCGAACATGAGTACGATTCGCTAAGCGATAATGACAAACGTCTCTTTGTCCGCCTGCTGGAAAATGATGATCCCGATT
TATTTAACTGGCTTATGAACCACGGCAAGCCACAGGATGCGGAGTTGCAAAGAATGGTGCAGTTAATCCAGACACGGAAT
CGGGATCGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3