Detailed information of TA system TA157442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 526885..527534
• Replicon: chromosome
• Accession: NZ_CP053682
• Organism: Proteus mirabilis strain MPE0156

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HNS39_RS02275
• Protein ID: WP_060556774.1
• Coordinates: 526885..527304 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: HNS39_RS02280
• Protein ID: WP_012368535.1
• Coordinates: 527301..527534 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNS39_RS02245	522161..523039	-	879	WP_060556778.1	23S rRNA pseudouridine(2604) synthase RluF	-
HNS39_RS02250	523215..524129	-	915	WP_060556777.1	fatty acid biosynthesis protein FabY	-
HNS39_RS02255	524153..524590	-	438	WP_060556776.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HNS39_RS02260	524671..525288	-	618	WP_060556775.1	glucose-1-phosphatase	-
HNS39_RS02265	525942..526358	-	417	WP_049196823.1	hypothetical protein	-
HNS39_RS02270	526336..526659	-	324	WP_060556812.1	hypothetical protein	-
HNS39_RS02275	526885..527304	-	420	WP_060556774.1	type II toxin-antitoxin system VapC family toxin	Toxin
HNS39_RS02280	527301..527534	-	234	WP_012368535.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HNS39_RS02285	528296..530131	-	1836	WP_004246802.1	ribosome-dependent GTPase TypA	-
HNS39_RS02290	530491..531900	+	1410	WP_004246801.1	glutamate--ammonia ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15383.94 Da
• Isoelectric Point: 7.7288

>T157442 WP_060556774.1 NZ_CP053682:c527304-526885 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNIREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T157442 NZ_CP069319:164253-164513 [Kosakonia cowanii]
ATGACGCCCAGGATAACGCCGGAAGCGCAAGCTGATATAGATTTCTGGAAGCAGAACGACCCTAAGGTAAAGCAGAAGAT
TGATACCCTCGTGAGGGATGCGCTTGAGCACCCTTTCCAGGGGCTCGGAAAGCCGGAGGCGCTTAAGCACCATAAGCCGC
TCTGGTCGCGCCGCATCACCAAAGAGCATCGACTCGTTTACTACATAAAAGATGACTCGCTGATTATCGTTTCTTGCCGC
TACCACTACGTAAATCTTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT157442 WP_012368535.1 NZ_CP053682:c527534-527301 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT157442 NZ_CP069319:164002-164256 [Kosakonia cowanii]
ATGAATATGCAACATGTTAACTACACCGACGCCCGCCAGAACCTGGCCTCGCTGATGCAGCAGGCAAATGACGAGCTGGC
ACCGATCCTTGTCACCCGCAAAGGGGCTAAAAGCGTGGTGGTTATCGATGCCGAGGAGTTTGCCTCTATGCAAGAGACGC
TTCATCTGTTCAGCAACCCGGTCAACGCCGCGCATATTGAGCGCAGCATCCAACAGGCGGAAAGCGGCGACCTACAGGAA
TATAAATTTGAATGA

Structures

Antitoxin

• AlphaFold DB: B4EZC0