Detailed information of TA system TA157436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1253080..1254101
• Replicon: chromosome
• Accession: NZ_CP053681
• Organism: Proteus mirabilis strain M3-1-17

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A8E4FRK4
• Locus tag: HNS37_RS05785
• Protein ID: WP_017827532.1
• Coordinates: 1253532..1254101 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: HNS37_RS05780
• Protein ID: WP_004245528.1
• Coordinates: 1253080..1253532 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNS37_RS05760	1248866..1249396	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
HNS37_RS05765	1249665..1250678	+	1014	WP_107033621.1	thiamine ABC transporter substrate binding subunit	-
HNS37_RS05770	1250678..1252258	+	1581	WP_060557657.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
HNS37_RS05775	1252251..1252952	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
HNS37_RS05780	1253080..1253532	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
HNS37_RS05785	1253532..1254101	+	570	WP_017827532.1	PIN domain-containing protein	Toxin
HNS37_RS05790	1254217..1256571	+	2355	WP_060557656.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21601.08 Da
• Isoelectric Point: 7.0180

>T157436 WP_017827532.1 NZ_CP053681:1253532-1254101 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T157436 NZ_CP069318:c1129635-1129252 [Delftia lacustris]
ATGTCCACCCAGCCGCGCAAACCGCTCAAATGGGTCGGCTCGGCCAAGCGGGATCTGGACGGAATGCCCGAGGACGTCCA
AGACGTGTTCGGCCATGCGATCGACCTGGCGCAAGCCGGTGGCAAGCACCCGGACGCCAAGGCGCTGAGCGGGTTTGGTT
CCGCCGCGGTTTTGGAAGTGGTCGAGGATTTCCGGAGCGACACGTTCCGGGCGGTCTACACGGTCAAGTTCGCGGGCTGG
GTCTATGTGCTGCACTGTTTTCAGAAGAAGTCCAAGAGCGGCATCAAGACGCCGAAGGAGGACCTGGACCTGATCAAGGC
ACGGCTGAAGGCTGCGGTGCAGGATTTTGAGGCTTGGCAAGCAAAGCAAGGAGTAAAGCGATGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT157436 WP_004245528.1 NZ_CP053681:1253080-1253532 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT157436 NZ_CP069318:c1129255-1128926 [Delftia lacustris]
ATGAGCCAGAACAGCCACATCACCATCGAAGAAGGCAGCACCAACGTCTACGCGGACCTGGGCTATGCCGACGCTGCGGA
AATGCAGCGCAAGTCGCAGCTCGCGGCTGAGATTGCCCGCGCCATCAAGGCGCGACGCCTGACGCAACAGGGAGCCGCCG
CGCTGCTGGAAATCGATCAGTCCAAGATCTCCCGCATCACGCGCGGCCAGTTTCGCGGGGTCAGCGAGGCGAAGCTGCTG
GAACTGGTGGCCAAGCTGGGGCATGACGTGAAGATCGTGGTCGGTCCAGTACGGCGGCGTGCCGGTAAGATCGAGCTGCA
GTTCGCCTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3