Detailed information of TA system TA157434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2319117..2319880
• Replicon: chromosome
• Accession: NZ_CP053676
• Organism: MAG: Ferrovum myxofaciens isolate S2.4

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HO274_RS11445
• Protein ID: WP_273119791.1
• Coordinates: 2319386..2319880 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: HO274_RS11440
• Protein ID: WP_273119788.1
• Coordinates: 2319117..2319389 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO274_RS11405 (HO274_11370)	2314204..2315271	+	1068	WP_273119777.1	IS1595 family transposase	-
HO274_RS11410 (HO274_11375)	2315382..2315957	-	576	WP_273119779.1	thioredoxin family protein	-
HO274_RS11415 (HO274_11385)	2316202..2317272	-	1071	WP_273119781.1	IS630 family transposase	-
HO274_RS11420 (HO274_11390)	2317463..2317627	+	165	Protein_2237	transposase domain-containing protein	-
HO274_RS11425 (HO274_11395)	2317775..2318017	+	243	WP_273119783.1	hypothetical protein	-
HO274_RS11430 (HO274_11400)	2318017..2318361	+	345	WP_273119785.1	endoribonuclease MazF	-
HO274_RS11435 (HO274_11405)	2318410..2318928	-	519	WP_273119786.1	transposase	-
HO274_RS11440 (HO274_11415)	2319117..2319389	+	273	WP_273119788.1	DUF1778 domain-containing protein	Antitoxin
HO274_RS11445 (HO274_11420)	2319386..2319880	+	495	WP_273119791.1	GNAT family N-acetyltransferase	Toxin
HO274_RS11450 (HO274_11425)	2320003..2321310	+	1308	WP_273119793.1	RecQ family ATP-dependent DNA helicase	-
HO274_RS11455 (HO274_11430)	2321228..2321827	-	600	WP_273119795.1	hypothetical protein	-
HO274_RS11460 (HO274_11435)	2321824..2322114	-	291	WP_273119797.1	nucleotidyltransferase domain-containing protein	-
HO274_RS11465 (HO274_11440)	2322176..2323324	-	1149	WP_273119799.1	glycosyltransferase family 87 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2106482..2343663	237181
	-	flank	IS/Tn	-	-	2316202..2317272	1070

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (26-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-86)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18008.96 Da
• Isoelectric Point: 9.5786

>T157434 WP_273119791.1 NZ_CP053676:2319386-2319880 [Ferrovum myxofaciens]
MSLQLTAPQPLVATHLLDGFECGEAVLDEWLKRRAMTNQLSGASRTFVVADQDGRVYGYYAMAAGAVSHPMATGLVRRNM
PDPVPVMVLARLAVEHRAQGIKLGASLLRDAVNRAVMVSRNAGVRALLVHALHDRAKEFYEHHGFRESPQHPMTLMLCLN
VVKG

Nucleotide

• Length: 495 bp

>T157434 NZ_CP069318:289583-289864 [Delftia lacustris]
ATGACGATCCAGTCATTCAAGTGCCCCGACACCGAGCAACTGTTCCAGCAGAGGCGGGTGCGTCGGTGGAGCAGCATTGA
AGTGGTTGCGCTGCGCAAGCTTCGGATGCTGCATGCCGCTCATGTGCTTCAGGACTTGAGGGTCCCGCCCGGAAACCGGC
TGGAGGCGCTACAGGGGGATCGCAAGGGGCAGCACAGCATCCGCATCAATGACCAGTGGCGCGTGTGCTTTGTGTGGACC
CATGAAGGCCCGAGTCAGCTGGAGATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9970.47 Da
• Isoelectric Point: 6.4508

>AT157434 WP_273119788.1 NZ_CP053676:2319117-2319389 [Ferrovum myxofaciens]
MRNAAINLRALPEQRNLIDHAATLLGKNRSDFMLEAACDKAQAVVLDQVFFNMDADKFTQFTAMLDAPPSHNPGLERLMT
VTAPWVNAKA

Nucleotide

• Length: 273 bp

>AT157434 NZ_CP069318:289907-290206 [Delftia lacustris]
ATGAACAAACCTTTGAACCTCCCCACGCCAGGCGAAATCCTGGCCACTGAATTTCTGGAGCCCATGGGCCTGACGCAATA
CCGCCTGGCCCAGGCCATCCATGTCCCGCAGACACGCATCGCGGCCATTGTGAAGAACGGCCGCTCCATCACGGCGGATA
CGGCGCTGCGGCTGGCTCGCTTCTTTGGCACGTCCGTGGAGTTCTGGATGAATCTGCAAAGCCAGTACGACGCCGATATC
GCGCGCGAGGCCATAGGCGACGATCTGGAGTCCATCGAGCCCTTTGCTCTGCAGGCTTGA